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首页> 外文期刊>Brain research >Distribution of tau protein kinase I/glycogen synthase kinase-3beta, phosphatases 2A and 2B, and phosphorylated tau in the developing rat brain.
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Distribution of tau protein kinase I/glycogen synthase kinase-3beta, phosphatases 2A and 2B, and phosphorylated tau in the developing rat brain.

机译：tau蛋白激酶I /糖原合酶激酶3β，磷酸酶2A和2B以及磷酸化tau在发育中的大鼠脑中的分布。

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摘要

When trying to elucidate the role played by tau protein kinase I/glycogen synthase kinase-3beta (TPKI/GSK-3beta) in tau phosphorylation, it is important to consider the balance that exists between the various kinases and phosphatases that are involved in vivo. We studied developmental changes in the expressions of TPKI/GSK-3beta and phosphatases 2A and 2B in rat brains using immunoblot analysis. The expression of the kinase peaked postnatally at days 8-11 and returned then to low level after 5 weeks. Phosphatase 2A showed a similar pattern, increasing postnatally until day 14 and decreasing thereafter. On the other hand, phosphatase 2B was undetectable at the juvenile stage, but later its presence increased rapidly to peak at 5 weeks after birth, after which it was maintained at high levels throughout the adult stage. Immunohistochemical studies using the PAP method revealed details of the distribution of TPKI/GSK-3beta. At postnatal days 3-21 both gray and white matter were immunoreactive. Later, after 5 weeks, the immunoreactivity became more restricted to the gray matter. The staining of tau phosphorylated at Ser 199, Ser 396, and Ser 413 followed mostly the pattern of the kinase distribution throughout all stages of development. These data, therefore, confirm that TPKI/GSK-3beta is expressed primarily in neurons and especially in neurites until postnatal day 21, whereafter the distribution is concentrated mostly in the cell soma and the proximal neurite region.
机译：当试图阐明tau蛋白激酶I /糖原合酶激酶3beta（TPKI / GSK-3beta）在tau磷酸化中的作用时，重要的是要考虑到体内涉及的各种激酶与磷酸酶之间的平衡。我们使用免疫印迹分析研究了大鼠脑中TPKI / GSK-3beta和磷酸酶2A和2B表达的发育变化。该激酶的表达在出生后第8-11天达到峰值，并在5周后恢复到低水平。磷酸酶2A表现出相似的模式，从出生后直到第14天增加，此后减少。另一方面，磷酸酶2B在幼年期未检测到，但后来它的存在迅速增加，在出生后5周达到高峰，此后在整个成年期均保持高水平。使用PAP方法进行的免疫组织化学研究揭示了TPKI / GSK-3beta分布的细节。出生后3-21天，灰色和白色物质均具有免疫反应性。后来，在5周后，免疫反应变得更加局限于灰质。 tau在199，Ser 396和Ser 413处磷酸化的染色在整个发育的所有阶段都遵循激酶分布的模式。因此，这些数据证实，直到出生后第21天，TPKI / GSK-3beta才主要在神经元中表达，尤其是在神经突中表达，此后分布主要集中在细胞体和近端神经突区域。

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