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首页> 外文期刊>Brain research >Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose.
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Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose.

机译：脱氢表雄酮抑制了缺乏葡萄糖的免疫刺激大鼠C6胶质瘤细胞的死亡。

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Pretreatment of interferon-gamma and lipopolysaccharides made C6 glioma cells highly vulnerable to glucose deprivation. Neither 12 h of glucose deprivation nor 2-day treatment with interferon-gamma (100 U/ml) and lipopolysaccharides (1 microg/ml) altered the viability of C6 glioma cells. However, significant death of immunostimulated C6 glioma cells was observed after 5 h of glucose deprivation. The augmented death was prevented by dehydroepiandrosterone (DHEA) treatment during immunostimulation, but not by DHEA treatment during glucose deprivation. DHEA reduced the rise in nitrotyrosine immunoreactivity, a marker of peroxynitrite, and superoxide production in glucose-deprived immunostimulated C6 glioma cells. DHEA, however, did not protect glucose-deprived C6 glioma cells from the exogenously produced peroxynitrite by 3-morpholinosydnonimine. Further, DHEA did not alter the production of total reactive oxygen species and nitric oxide in immunostimulated C6 glioma cells. Superoxide dismutase (SOD) and the synthetic SOD mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin inhibited the death of glucose-deprived immunostimulated C6 glioma cells. In addition, a superoxide anion generator paraquat reversed the protective effect of DHEA on the augmented death. The data indicate that DHEA prevents the glucose deprivation-evoked augmented death by inhibiting the production of superoxide anion in immunostimulated C6 glioma cells.
机译：干扰素-γ和脂多糖的预处理使C6胶质瘤细胞极易受到葡萄糖剥夺的影响。剥夺葡萄糖12小时或用干扰素-γ（100 U / ml）和脂多糖（1 microg / ml）进行2天治疗都不会改变C6胶质瘤细胞的生存能力。然而，在葡萄糖剥夺5小时后，观察到免疫刺激的C6胶质瘤细胞大量死亡。在免疫刺激过程中，通过脱氢表雄酮（DHEA）治疗可预防增加的死亡，而在葡萄糖剥夺期间，可通过DHEA治疗无法预防。 DHEA减少了硝基酪氨酸免疫反应性（过氧亚硝酸盐的标志物）的升高，并减少了葡萄糖缺乏的免疫刺激的C6胶质瘤细胞中的过氧化物生成。 DHEA，但是，不能保护葡萄糖缺乏的C6胶质瘤细胞免受3-吗啉代亚砜亚胺外源产生的过氧亚硝酸盐的影响。此外，DHEA不会改变免疫刺激的C6胶质瘤细胞中总活性氧和一氧化氮的产生。超氧化物歧化酶（SOD）和合成的SOD模拟锰（III）四（4-苯甲酸）卟啉抑制葡萄糖缺乏免疫刺激的C6胶质瘤细胞的死亡。此外，超氧化物阴离子产生剂百草枯逆转了DHEA对增加死亡的保护作用。数据表明，DHEA通过抑制免疫刺激的C6胶质瘤细胞中超氧阴离子的产生，防止了葡萄糖剥夺引起的增加的死亡。

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