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首页> 外文期刊>Brain research >Neuronal expression of Fos protein in the hypothalamus of rats with heart failure.
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Neuronal expression of Fos protein in the hypothalamus of rats with heart failure.

机译：心力衰竭大鼠下丘脑Fos蛋白的神经元表达。

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We sought to identify the areas that have altered neuronal activity within the hypothalamus of rats with heart failure (HF) by mapping neuronal staining of c-Fos protein (Fos) 6-8 weeks following coronary artery ligation (HF group; n=17) or sham surgery (sham-operated control group, n=15). Fos-like immunoreactivity was observed in the paraventricular nucleus (PVN), supraoptic nucleus (SON), median preoptic nucleus (MnPO), anterior hypothalamus (AH) and posterior hypothalamus (PH) using a standard ABC immunocytochemical protocol. The rats in the HF group displayed infarcts averaging 34+/-2% of the outer circumference and 41+/-1% of the inner circumference of the left ventricular wall. Sham-operated control rats had no observable damage to the myocardium. Rats with chronic heart failure (n=5) but no manipulation (no surgery) had a similar number of Fos-staining cells in PVN SON, MnPO, AH and PH compared to sham-operated rats. Acute surgery for isolation of vagus nerves and anesthesia for 90 min increased the number of Fos positive cells in PVN, SON and MnPO of both sham-operated rats and rats with HF. Furthermore, rats with heart failure (n=5) had significantly higher number of Fos-staining cells in PVN (four times), SON (4.5 times) and MnPO (1.5 times) compared to sham-operated rats after acute surgery for isolation of the vagus. The number of Fos-staining cells remained unaltered in AH and PH in both groups of rats. However, in a third series of experiments vagotomy reduced the number of Fos-staining cells in the PVN, SON or MnPO of rats with HF (n=5) to those observed in sham-operated vagotomized rats. This study shows that: (1) there is augmented neuronal activity as indicated by increased number of Fos staining neurons in the PVN, SON and MnPO due to acute surgical stress in rats with HF, and (2) vagal afferents are responsible for the increased neuronal activity in PVN, SON and MnPO of rats with HF during acute surgical stress. These data support the conclusion that vasopressin producing neurons and autonomic areas within the hypothalamus influenced by vagal afferents are activated during HF and are sensitive to 'acute surgical stress' and may contribute to the elevated levels of vasopressin and sympatho-excitation commonly observed in heart failure.
机译：我们试图通过绘制冠状动脉结扎后6-8周的c-Fos蛋白（Fos）的神经元染色图来确定心力衰竭（HF）大鼠下丘脑神经元活性发生改变的区域（HF组; n = 17）或假手术（假手术对照组，n = 15）。使用标准的ABC免疫细胞化学方法，在室旁核（PVN），视上核（SON），正视前核（MnPO），下丘脑前部（AH）和后丘脑后部（PH）中观察到Fos样免疫反应。 HF组大鼠的梗塞平均占左心室壁外周的34 +/- 2％和内周的41 +/- 1％。假手术的对照大鼠对心肌没有可观察到的损伤。与假手术大鼠相比，患有慢性心力衰竭（n = 5）但不进行操作（不进行手术）的大鼠在PVN SON，MnPO，AH和PH中的Fos染色细胞数量相似。假手术迷走神经和麻醉的急性手术90分钟增加了假手术大鼠和HF大鼠的PVN，SON和MnPO中Fos阳性细胞的数量。此外，与假手术分离后的假手术大鼠相比，心力衰竭大鼠（n = 5）在PVN中的Fos染色细胞数量明显增加（四倍），SON（4.5倍）和MnPO（1.5倍）。迷走神经两组大鼠的AH和PH中Fos染色细胞的数量保持不变。然而，在第三系列实验中，迷走神经切断术将具有HF（n = 5）的HF大鼠的PVN，SON或MnPO中的Fos染色细胞的数量减少了，与假手术的迷走神经切断的大鼠中观察到的数量相比。这项研究表明：（1）HF大鼠因急性外科手术应激，PVN，SON和MnPO中Fos染色神经元数量增加表明神经元活性增强;（2）迷走神经传入负责增加急性外科应激状态下HF大鼠神经元PVN，SON和MnPO的神经活动这些数据支持以下结论：受迷走神经传入影响的下丘脑中产生加压素的神经元和自主神经区域在HF期间被激活，并且对“急性手术压力”敏感，并且可能导致心力衰竭中通常观察到的加压素和交感兴奋水平升高。

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