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首页> 外文期刊>Brain research >Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons.
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Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons.

机译：P物质和降钙素基因相关肽对成年小鼠背根神经节神经元轴突运输的影响。

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摘要

Substance P and calcitonin gene-related peptide (CGRP) released from primary sensory neurons are known to play important roles in nociception and nociceptive transmission. In the present study, we attempted to clarify the roles of these neuropeptides in the regulation of axonal transport in sensory neurons. Cells were isolated from adult mouse dorsal root ganglia and cultured in F-12 medium containing fetal bovine serum for 48 h until their neurites were grown. These isolated and cultured DRG cells were mostly (>98%) small (diameter <25 microm) and medium (diameter, 25-40 microm) in size, and were immunoreactive for substance P and CGRP (85.9 and 66. 0% of total cells, respectively). Video-enhanced microscopy was applied to observe particles transported within neurites. Application of substance P (100 nM) decreased the number of particles transported in both anterograde and retrograde directions in each of DRG neurons tested (n=5). The instantaneous velocities of individual particles transported in anterograde and retrograde directions were also reduced by substance P. In contrast, alpha-CGRP (100 nM) increased the number of particles transported in both directions in each of DRG neurons tested (n=5), and also increased the instantaneous velocities of particles transported bidirectionally. Application of beta-CGRP (100-1000 nM) did not elicit any effect on axonal transport. Therefore, axonal transport in sensory neurons seems to be modulated by substance P and alpha-CGRP, both of which can be derived from its own and adjacent sensory neurons.
机译：从初级感觉神经元释放的P物质和降钙素基因相关肽（CGRP）在伤害感受和伤害感受传递中起重要作用。在本研究中，我们试图阐明这些神经肽在调节感觉神经元轴突运输中的作用。从成年小鼠背根神经节中分离细胞，并在含有胎牛血清的F-12培养基中培养48小时，直到其神经突生长。这些分离和培养的DRG细胞大部分（> 98％）小（直径<25微米），中等（直径25-40微米），对P物质和CGRP具有免疫反应性（分别为85.9和66. 0％）单元格）。应用视频增强显微镜观察神经突内转运的颗粒。施用P物质（100 nM）减少了测试的每个DRG神经元在顺行和逆行方向上运输的颗粒数量（n = 5）。物质P也降低了沿顺行和逆行方向运输的单个颗粒的瞬时速度。相反，α-CGRP（100 nM）增加了每个测试的DRG神经元在两个方向上运输的颗粒数量（n = 5），并且还增加了双向传输粒子的瞬时速度。使用β-CGRP（100-1000 nM）不会对轴突运输产生任何影响。因此，感觉神经元中的轴突运输似乎受到物质P和α-CGRP的调节，这两种物质都可以来自其自身和邻近的感觉神经元。

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