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首页> 外文期刊>Brain research >Glutamic acid decarboxylase-immunoreactivity of bulbar respiratory neurons identified by intracellular recording and labeling in rats.
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Glutamic acid decarboxylase-immunoreactivity of bulbar respiratory neurons identified by intracellular recording and labeling in rats.

机译：通过大鼠细胞内记录和标记鉴定的延髓呼吸神经元的谷氨酸脱羧酶免疫反应性。

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摘要

To distinguish the GABAergic neuron in the ventral respiratory group (VRG) of rats, immunohistochemical staining of glutamic acid decarboxylase (GAD) was performed in neurons that had been individually identified by in vivo intracellular recording and labeling with neurobiotin. A total of five types of respiratory neurons were identified and labeled; augmenting inspiratory (aug-I, n=12), decrementing or early inspiratory (early-I, n=3), inspiration-expiration phase spanning or late inspiratory (late-I, n=3), decrementing expiratory or postinspiratory (PI, n=8), and augmenting or stage 2 expiratory (E2, n=3). In addition, expiration-inspiration phase-spanning or pre-inspiratory neurons (pre-I, n=2) were recorded, but not labeled. The membrane potential trajectory of each neuron type resembled that previously described in cat, suggesting a comparable neuronal organization between the two species. According to the axonal arborization, those labeled neurons were further classified as propriobulbar (6 aug-I, all early-I, all late-I, and 3 PI), bulbospinal (2 aug-I and all E2) and cranial-motor neurons (4 aug-I and 5 PI). GAD-immunoreactivity was consistently detected in the propriobulbar neurons, while it was not seen in cranial-motor and bulbospinal neurons. In addition, GAD-immunoreactive varicosities were found surrounding the somatic and dendritic surface of all labeled neurons. The present results illustrate that the propriobulbar types of early-I, aug-I, late-I and PI neurons are GABAergic inhibitory neurons and virtually all types of respiratory neurons receive GABAergic inputs in the rat's VRG.
机译：为了区分大鼠腹侧呼吸组（VRG）中的GABA能神经元，对神经元进行了谷氨酸脱羧酶（GAD）的免疫组织化学染色，这些神经元已通过体内细胞内记录和神经生物素标记进行了单独鉴定。总共识别并标记了五种呼吸神经元;吸气增加（aug-I，n = 12），递减或早期吸气（early-I，n = 3），吸气-呼气阶段跨越或吸气末期（-I，n = 3），递减呼气或吸气后（PI） ，n = 8），并增加或进入第2阶段呼气（E2，n = 3）。此外，记录了呼气-吸气阶段跨度或吸气前神经元（pre-I，n = 2），但未标记。每种神经元类型的膜电位轨迹类似于先前在猫中描述的轨迹，表明这两个物种之间具有类似的神经元组织。根据轴突，将那些标记的神经元进一步分为：球房神经（6 aug-I，所有早期-I，所有晚期-I和3 PI），球颈（2 aug-I和所有E2）和颅运动神经元（4月1日至5日）。 GAD免疫反应性一直在球前神经元中被检测到，而在颅运动神经元和球茎神经元中未见到。此外，在所有标记神经元的体细胞和树突表面周围发现了GAD免疫反应性静脉曲张。目前的结果表明，早期I，aug-I，晚期I和PI神经元的球房类型是GABA能抑制神经元，并且几乎所有类型的呼吸神经元都在大鼠VRG中接受GABA能输入。

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