A single space polymerase chain reaction for combined gene detection andepidemiological typing of Listeria monocytogenes

Medeiros D; Farber JM

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A single space polymerase chain reaction for combined gene detection andepidemiological typing of Listeria monocytogenes

机译：A single space polymerase chain reaction for combined gene detection andepidemiological typing of Listeria monocytogenes

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摘要

A variant of the random amplification of polymorphic DNA (RAPD) technique, referred to as the 'combined gene detection and epidemiological typing' (COGEDET) method was employed in the development of a one-step PCR assay for simultaneous identification and typing of Listeria monocytogenes. An arbitrary (RAPD) primer was used in combination with a species specific gene primer set. Four gene primers, HLYA, PRFA, ISP and FL2A, and six RAPD primers were screened for their discriminatory abilities.

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来源
《Food Microbiology》 |2001年第4期|375-386|共12页
作者
Medeiros D; Farber JM;
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作者单位

Farber JM, Hlth Canada, Microbial Res Div, Bur Microbial Hazards, Food Directorate, Postal Locator 2204A2, Ottawa, ON K1A 0L2, Canada;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类食品工业;
关键词
Polymorphic dna rapd; Field gel-electrophoresis; Random amplification.; Escherichia-coli; Identification; Food; Pcr; Strains; Outbreak.; Probe;

A single space polymerase chain reaction for combined gene detection andepidemiological typing of Listeria monocytogenes

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