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首页> 外文期刊>Infection control and hospital epidemiology >Use of a novel selective medium to detect methicillin-resistant Staphylococcus aureus in colonized patients of an intensive care unit.
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Use of a novel selective medium to detect methicillin-resistant Staphylococcus aureus in colonized patients of an intensive care unit.

机译：新型选择性培养基在重症监护病房定植患者中检测耐甲氧西林金黄色葡萄球菌的用途。

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BACKGROUND: Detection of colonized patients is important for implementing control measures for methicillin-resistant Staphylococcus aureus (MRSA). Laboratory detection of MRSA carriers is increased by the use of selective screening media, helping control dissemination of such organisms. OBJECTIVE: To evaluate three different media, including selective and nonselective media, in the detection of MRSA from clinical specimens of patients of an intensive care unit (ICU). PATIENTS: Adult patients in the ICU of the Hospital Mae de Deus, Porto Alegre, Brazil. METHODS: A total of 224 specimens were obtained from the nares of patients and plated on blood agar, mannitol salt agar containing 2 microg/mL of oxacillin (MSAO), and oxacillin resistance screening agar base (ORSAB). The presence of MRSA was investigated in typical colonies growing on the three types of media. Discrepant results were resolved by detection of the mecA gene by polymerase chain reaction and the modified penicillin binding protein known as PBP2'. RESULTS: MRSA was detected in 32 (14.3%) of 224 specimens. Blood agar, MSAO, and ORSAB detected MRSA in 17, 31, and 28 specimens, respectively. After the coagulase test, no false resistance was observed. CONCLUSION: MSAO and ORSAB performed equivalently in the detection of MRSA of colonized patients and require a single supplementary test (coagulase) to confirm MRSA.
机译：背景：定植患者的检测对于实施耐甲氧西林金黄色葡萄球菌（MRSA）的控制措施很重要。通过使用选择性筛选介质，可以增加实验室对MRSA载体的检测，从而有助于控制此类生物的传播。目的：评估重症监护病房（ICU）患者临床标本中MRSA检测中三种不同的介质，包括选择性和非选择性介质。患者：巴西阿雷格里港Mae de Deus医院ICU的成年患者。方法：从患者的鼻孔中获得224份标本，并将其涂在血琼脂，含2微克/毫升奥沙西林（MSAO）的甘露醇盐琼脂和奥沙西林耐药性筛选琼脂基质（ORSAB）上。在三种类型培养基上生长的典型菌落中研究了MRSA的存在。通过聚合酶链反应和修饰的青霉素结合蛋白（称为PBP2'）检测mecA基因，可以解决不一致的结果。结果：在224个样本中有32个（14.3％）检测到MRSA。血琼脂，MSAO和ORSAB分别在17、31和28个样本中检测到MRSA。凝固酶测试后，未观察到假抗性。结论：MSAO和ORSAB在定植的患者的MRSA检测中表现相当，并且需要进行一次补充检测（凝固酶）以确认MRSA。

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