Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal.

Suzuki T; Abe Y; McGehee DS; Keath JR; Yajima H; Sharma K; Brorson JR

首页> 外文期刊>Brain research. Brain research protocols >Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal.

【24h】

Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal.

机译：活体动物皮质脊髓神经元的长期逆行荧光标记。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

For pathophysiological studies, it is advantageous to label specific neuronal populations in living animals. This study aimed to establish a method for stable and long-lasting fluorescent labeling of corticospinal neurons in the living animal. The two fluorescent dyes Fluoro-Red and Fluoro-Green were injected in the cervical spinal cord of anesthetized newborn rats. After a recovery period, treated rats were returned to the mother. After 24 h and 14 days, fixed brain sections revealed wide-spread fluorescence in elongated or pyramidal-shaped cell profiles in a discrete internal cortical layer, consistent with layer V pyramidal cells. Labeled neurons displayed spontaneous synaptic activity using the slice patch clamp method. These results suggest that these dyes are effective tools for pathophysiological and slice patch clamp studies focused on specific neuron groups.

机译：对于病理生理研究，在活体动物中标记特定的神经元种群是有利的。这项研究旨在建立一种在活体动物中对皮质脊髓神经元进行稳定和持久荧光标记的方法。将两种荧光染料Fluoro-Red和Fluoro-Green注入麻醉的新生大鼠的颈脊髓中。恢复期后，将治疗的大鼠送回母亲。 24小时和14天后，固定的大脑切片在不连续的内部皮质层中与V层锥体细胞一致，在细长或金字塔形的细胞轮廓中显示出广泛的荧光。标记的神经元使用切片膜片钳方法显示自发的突触活性。这些结果表明，这些染料是针对特定神经元组进行病理生理学和切片膜片钳研究的有效工具。

著录项

来源
《Brain research. Brain research protocols》 |2004年第3期|共6页
作者
Suzuki T; Abe Y; McGehee DS; Keath JR; Yajima H; Sharma K; Brorson JR;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类神经病学与精神病学;
关键词
livin; g lt; 3gt; Animals; patch clamp; 动物;

机译：livin;g 3;Animals;patch clamp;动物;

相似文献

外文文献
中文文献
专利

1. Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal. [J] . Suzuki T, Abe Y, McGehee DS, Brain research. Brain research protocols . 2004,第3期

机译：活体动物皮质脊髓神经元的长期逆行荧光标记。
2. Expression of L1 and TAG-1 in the corticospinal, callosal, and hippocampal commissural neurons in the developing rat telencephalon as revealed by retrograde and in situ hybridization double labeling. [J] . Fujimori KE, Takeuchi K, Yazaki T, The Journal of Comparative Neurology . 2000,第3期

机译：逆行和原位杂交双重标记揭示了发育中大鼠端脑的皮质脊髓，call骨和海马连合神经元中L1和TAG-1的表达。
3. Retrograde labeling of primary sensory neurons with fluorescent latex microspheres: a useful tool for long term tagging of neurons. [J] . Altun M, Bergman E, Ulfhake B Journal of Neuroscience Methods . 2001,第1期

机译：荧光乳胶微球逆行标记初级感觉神经元：长期标记神经元的有用工具。
4. Real-time imaging of dynamic translocation of fluorescent proteins at synapses in living neurons [C] . Paul De Koninck Fifth International Conference on Applications of Photonic Technology (ICAPT 2002), Jun 1-6, 2002, Quebec City . 2002

机译：活体神经元突触中荧光蛋白动态易位的实时成像
5. eGFP expression under the Uchl1 promoter labels corticospinal motor neurons and a subpopulation of degeneration resistant spinal motor neurons in ALS mouse models [D] . Yasvoina, Marina V. 2013

机译：UCH11启动子标记下的EGFP表达皮质脊髓运动神经元和ALS小鼠模型中变性抗性脊柱运动神经元的亚群
6. Retrograde labeling transduction and genetic targeting allow cellular analysis of corticospinal motor neurons: implications in health and disease [O] . Javier H. Jara, Barış Genç, Jodi L. Klessner, 2014

机译：逆行标记转导和基因靶向可以对皮质脊髓运动神经元进行细胞分析：对健康和疾病的影响
7. Retrograde labeling, transduction and genetic targeting allow cellular analysis of corticospinal motor neurons: Implications in health and disease. [O] . Javier Hernan Jara, Baris eGenc, Jodi L Klessner, 2014

机译：逆行标记，转导和遗传靶向允许皮质脊髓运动神经元的细胞分析：对健康和疾病的影响。

Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal.

摘要

著录项

相似文献

相关主题

期刊订阅