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Polyphenols, antioxidant and antimicrobial activities of leaf and bark extracts of Solidago canadensis L.

机译:加拿大一枝黄花叶和树皮提取物的多酚,抗氧化剂和抗菌活性。

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摘要

The leaf and bark of Solidago canadensis L. (SCL) contain a wide range of bioactive compounds that may be responsible for their biological activities. However, little information is available about their specific active compounds, as well as their contributions to the antioxidant and antimicrobial properties. This study investigated the total phenolics, tannins and flavonoid contents and antioxidant and antimicrobial activities of high pressure (HPE) or ultrasound CUE) assisted ethanolic extracts from leaf and bark of SCL at three ripening stages (vegetative growth (VG), full bloom (FB) and maturity after flowering (MF)). The antioxidant activities and contents of total phenolic (TPC), tannins (TT'C) and flavonoids (TFC) varied with ripeness stage, tissue type and extraction method. Overall, the UE leaf extracts at the FB stage exhibited the highest TPC (3.8 mg GAE/g DM), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity (0.547 mg AAE/g DM) and oxygen radical absorbing capacity (ORAC) value (57.86 mmol TE/g DM). The HPE extracts of MF samples had the greatest TFC (2.45 mg RE/g DM) and reducing power (3.38). The greatest TTC (4.17 g/100 g DM) was observed in the ethanolic extracts at the MF stage. All leaf extracts exhibited antimicrobial activity against Listeria monocytogenes and Staphylococcus aureus, but only the HPE extracts of the VG samples showed activity against Salmonella spp. The UE leaf extracts at the MF stage demonstrated the maximum inhibitory potency against Escherichia coil, L. monocytogenes and S. aureus. These results highlighted the potential of using S. canadensis extracts as natural antimicrobial and antioxidant substances for food applications. (C) 2015 Elsevier B.V. All rights reserved.
机译:加拿大一枝黄花(SCL)的叶和树皮含有多种生物活性化合物,可能与其生物活性有关。然而,关于它们的特定活性化合物以及它们对抗氧化剂和抗微生物特性的贡献的信息很少。这项研究调查了三个成熟阶段(营养生长(VG),盛开(FB))的SCL叶片和树皮中的高压(HPE)或超声波CUE辅助乙醇提取物的总酚,单宁和类黄酮含量以及抗氧化和抗菌活性。 )和开花后的成熟度(MF)。总酚,总丹宁(TT'C)和类黄酮(TFC)的抗氧化活性和含量随成熟阶段,组织类型和提取方法而变化。总体而言,在FB阶段,UE叶提取物表现出最高的TPC(3.8 mg GAE / g DM),2,2-二苯基-1-吡啶并肼基(DPPH)清除能力(0.547 mg AAE / g DM)和氧自由基吸收能力(ORAC)值(57.86 mmol TE / g DM)。 MF样品的HPE提取物具有最大的TFC(2.45 mg RE / g DM)和还原力(3.38)。在MF阶段,在乙醇提取物中观察到最大的TTC(4.17 g / 100 g DM)。所有叶提取物均表现出对单核细胞增生李斯特菌和金黄色葡萄球菌的抗菌活性,但仅VG样品的HPE提取物对沙门氏菌具有活性。 UE叶提取物在MF阶段表现出对大肠杆菌,单核细胞增生李斯特菌和金黄色葡萄球菌的最大抑制效力。这些结果突显了将加拿大链球菌提取物用作食品应用中的天然抗菌和抗氧化剂的潜力。 (C)2015 Elsevier B.V.保留所有权利。

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