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首页> 外文期刊>Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases >High-throughput RNA sequencing profiles and transcriptional evidence of aerobic respiratory enzymes in sporulating oocysts and sporozoites of Eimeria tenella
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High-throughput RNA sequencing profiles and transcriptional evidence of aerobic respiratory enzymes in sporulating oocysts and sporozoites of Eimeria tenella

机译：艾美球虫卵囊和子孢子中有氧呼吸酶的高通量RNA测序和转录证据

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摘要

Seven species of Eimeria are responsible for coccidiosis in chickens. Eimeria tenella is one of the most pathogenic parasites since it is associated with high mortality and great economic impact. The life cycle of the parasite includes development in the environment and in the intestinal tract. We conducted RNA sequencing using a next generation sequencer to obtain transcriptome information from the sporulating oocysts, and sporozoites. We collected 2.8 million 75 bp reads of a short-tag sequence, and 25,880 contigs were generated by the Oases assembler. A Blastx search of GenBank databases revealed that 7780 contigs (30.1%) had significant homology with deposited sequence data (E-value < 1e-6); among these contigs, 6051 contigs were similar to those of Toxoplasma gondii while only 513 contigs (6.6%) were similar to those of E. tenella. After an orthological analysis conducted with the UniProt database of T. gondii, 6661 contigs were distributed within the categories of cellular components (1528 gene categories), biological processes (861 gene categories), and molecular functions (241 gene categories). The significantly matched contigs contained high numbers of enzymes associated with glycolysis, TCA, and the pentose-phosphate pathway. Most of the enzymes, measured by quantitative reverse transcription-PCR, were up-regulated in sporulating stage. These results suggest that the intracellular carbohydrate amylopectin could be used as an energy source for ATP production including glycolysis and the pentose-phosphate pathway, which generates NADPH and pentoses. Our data also suggest that Eimeria might possess a partial or similar pathway to the TCA cycle essential for aerobic respiration. Furthermore, the newly annotated and non-annotated contigs might contain E. tenella-specific or novel sequences
机译：艾美球虫有7种是造成鸡球虫病的原因。艾美球虫是致病性最强的寄生虫之一，因为它具有很高的死亡率和巨大的经济影响。寄生虫的生命周期包括环境和肠道中的发育。我们使用下一代测序仪进行RNA测序，以从孢子形成的卵囊和子孢子获得转录组信息。我们收集了一个短标签序列的280万个75 bp读段，Oases汇编程序产生了25,880个重叠群。对GenBank数据库的Blastx搜索显示，有7780个重叠群（30.1％）与存放的序列数据具有显着同源性（E值<1e-6）;在这些重叠群中，有6051个重叠群与弓形虫相似，而只有513个重叠群（6.6％）与E. tenella相似。经过对弓形虫的UniProt数据库进行的正交分析后，在细胞成分（1528个基因类别），生物学过程（861个基因类别）和分子功能（241个基因类别）类别中分布了6661个重叠群。显着匹配的重叠群含有与糖酵解，TCA和戊糖-磷酸途径相关的大量酶。通过定量逆转录PCR测量的大多数酶在孢子形成阶段被上调。这些结果表明，细胞内碳水化合物支链淀粉可以用作产生ATP的能量来源，包括糖酵解和产生NADPH和戊糖的戊糖-磷酸途径。我们的数据还表明，艾美球虫可能具有部分或类似途径，成为有氧呼吸必不可少的TCA周期。此外，新注释和未注释的重叠群可能包含特异大肠杆菌的序列

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