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首页> 外文期刊>Industrial Crops and Products >Improving bioactivities of polyphenol extracts from Psidium guajava L. leaves through co-fermentation of Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139
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Improving bioactivities of polyphenol extracts from Psidium guajava L. leaves through co-fermentation of Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139

机译：通过共同发酵红曲霉GIM 3.592和酿酒酵母GIM 2.139改善番石榴叶中多酚提取物的生物活性。

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Although phenolic compounds linked to proteins or polysaccharides in the cell wall from herbal plant through beta-glycoside bonds are difficult to extract in aqueous solution, microorganisms that produce some key enzyrnes may be useful for enhancing the release of phenolic compounds. Solid-state fermentation with a combination of different microorganisms was used to promote the bioactivities of the polyphenol component in GL. The results showed that co-fermentation of GL with Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139 led to a significant increase in contents of quercetin and total polyphenols. Including gallic acid, chlorogenic acid, rutin, isoquercitrin, avicularin, quercitrin, kaempferol, and quercetin, all polyphenols extracted, from fermented guava leaves (FGL) were quantitatively identified by high-performance liquid chromatography time-of-flight electrospray ionization mass spectrometry (HPLC-TOF-ESI/MS). Quercetin, which presented the highest bioactivity, was significantly increased after microbial conversion. Total polyphenols extracted from FGL showed higher bioactivities in scavenging 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) caption radicals, anti-bacterial activity, and alpha-glucosidase inhibitory activity than those from GL. The activities of total polyphenols extracted from FGL by methanol were determined by calculating the IC50, with the highest values found to be 30.5711 mu g/mL, 24.53 mu g/mL, and 0.09 mu g/mL for the scavenging of DPPH and ABTS(+) radicals and inhibition of alpha-glucosidase, respectively. Methanol was more effective for extracting components with high alpha-glucosidase inhibitory activities than acetone. The methanol extract (0.20 mg/mL) efficiently inhibited 92.05% of alpha-glucosidase activity, which was significantly higher than that obtained using acarbose solution at an equal concentration. This microbial co-fermentation method can be used to upgrade the nutritional or medicinal values of herb leaves efficiently. (C) 2016 Elsevier B.V. All rights reserved.
机译：虽然通过水溶液很难提取通过β-糖苷键与草药植物细胞壁中的蛋白质或多糖连接的酚类化合物，但产生某些关键酶的微生物可用于增强酚类化合物的释放。结合不同微生物进行的固态发酵用于促进GL中多酚成分的生物活性。结果表明，GL与红曲霉GIM 3.592和啤酒酵母GIM 2.139共同发酵导致槲皮素和总多酚含量显着增加。包括高效液相色谱-飞行时间电喷雾电离质谱法（FLU）对从番石榴叶（FGL）中提取的所有多酚进行了定量鉴定，包括没食子酸，绿原酸，芦丁，异槲皮苷，鸟苷，槲皮素，山奈酚和槲皮素。 HPLC-TOF-ESI / MS）。表现出最高生物活性的槲皮素在微生物转化后显着增加。从FGL中提取的总多酚在清除1,1-二苯基-2-吡啶并肼基（DPPH）和2,2-叠氮基双3-乙基苯并噻唑啉-6-磺酸二铵盐（ABTS）标题自由基方面具有较高的生物活性，具有抗菌活性和GL的α-葡萄糖苷酶抑制活性。通过计算IC50确定甲醇从FGL中提取的总多酚的活性，发现其清除DPPH和ABTS的最高值为30.5711μg/ mL，24.53μg/ mL和0.09μg/ mL（ +）自由基和对α-葡萄糖苷酶的抑制。甲醇比丙酮更有效地提取具有高α-葡萄糖苷酶抑制活性的成分。甲醇提取物（0.20 mg / mL）有效抑制92.05％的α-葡萄糖苷酶活性，该活性明显高于使用相同浓度的阿卡波糖溶液所获得的活性。这种微生物共发酵方法可用于有效提高草本植物叶片的营养或药用价值。 （C）2016 Elsevier B.V.保留所有权利。

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