...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain research. Brain research protocols >A fluorescence-based method to assess plasma protein extravasation in rat dura mater using confocal laser scanning microscopy.
【24h】

A fluorescence-based method to assess plasma protein extravasation in rat dura mater using confocal laser scanning microscopy.

机译：基于荧光的方法使用共聚焦激光扫描显微镜评估大鼠硬脑膜中血浆蛋白的外渗。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a nonradioactive, fluorescence-based method to assess plasma protein extravasation (PPE) in rat dura mater using confocal laser scanning microscopy (CLSM). Unilateral PPE can be induced by electrical stimulation of the ipsilateral trigeminal ganglion (TG) and is widely used as an experimental migraine model. The gold standard to determine PPE in the meninges is based on the detection of radiolabeled albumin ([125]I-BSA). The aim of this study was to develop a nonradioactive, histological method to quantify PPE in the meninges. The fluorescent dye Evans Blue (50 mg/kg) was injected intravenously to the rat 7 min prior to TG stimulation. PPE in dura mater was detected by a CLSM. The amount of extravasated Evans Blue in the dura mater was measured at six to eight regions of interest (ROIs) in the vicinity of large meningeal vessels. The ratio of the average fluorescence intensity within dura mater of the stimulus side calculated for each animal. By using this method, The PPE ratio was 1.67+/-0.12 (n=5). Intravenous injection of three different dosages of the 5HT(1B/1D)-receptor agonist sumatriptan (25, 50, and 100 microg/kg) 15 min prior to stimulation attenuated PPE by 42+/-12%, 49+/-9%, and 86+/-15%, respectively (p<0.01). The approximated ED(50) value was 48 microg/kg. Our results are in accordance with previous reports in the literature using the radioactive approach. We conclude that CLSM is a safe, sensitive, and reliable method to assess PPE in rat meninges in an experimental migaine model.
机译：我们描述了一种非放射性的，基于荧光的方法，使用共聚焦激光扫描显微镜（CLSM）评估大鼠硬脑膜中的血浆蛋白外渗（PPE）。单侧PPE可以通过电刺激同侧三叉神经节（TG）来诱导，并被广泛用作实验性偏头痛模型。测定脑膜中PPE的金标准是基于放射性标记白蛋白（[125] I-BSA）的检测。这项研究的目的是开发一种非放射性的组织学方法来定量脑膜中的PPE。在TG刺激前7分钟，将荧光染料Evans Blue（50 mg / kg）静脉内注射给大鼠。 CLSM检测到硬脑膜中的PPE。在大脑膜血管附近的六到八个感兴趣区域（ROI）处测量了硬脑膜中渗出的伊文思蓝的量。计算每只动物的刺激侧硬脑膜内平均荧光强度的比率。通过使用该方法，PPE比为1.67 +/- 0.12（n ＝ 5）。刺激前15分钟静脉注射三种不同剂量的5HT（1B / 1D）-受体激动剂舒马曲坦（25、50和100 microg / kg），使PPE降低42 +/- 12％，49 +/- 9％和分别为86 +/- 15％（p <0.01）。 ED（50）的近似值是48微克/千克。我们的结果与以前使用放射​​性方法的文献报道一致。我们得出结论，CLSM是一种在实验性偏头痛模型中评估大鼠脑膜中PPE的安全，灵敏和可靠的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号