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首页> 外文期刊>INFORM: International News on Fats, Oils and Related Materials >Simultaneous measurement of retinol,alpha-tocopherol and six carotenoids in human plasma by using an isocratic reversed-phase HPLC method
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Simultaneous measurement of retinol,alpha-tocopherol and six carotenoids in human plasma by using an isocratic reversed-phase HPLC method

机译：等度反相HPLC法同时测定人血浆中的视黄醇，α-生育酚和六种类胡萝卜素

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摘要

A simple and sensitive isocratic reversed-phase high-performance liquid chromatography(HPLC)method for simultaneous determination of retinol,a-tocoph-erol and six carotenoids in human plasma was described.Sample preparation of the earlier published method was further developed by addition of ultrapure water,which enabled aqueous layer to freeze facilitating phase separation without pipetting thus also improving precision of the method.Developed method appeared to be less laborious and time consuming compared with the traditional extraction methods,which require removal of organic layer by pipetting.
机译：介绍了一种简单灵敏的等度反相高效液相色谱（HPLC）方法，可同时测定人血浆中的视黄醇，α-生育酚和六种类胡萝卜素。超纯水可以使水层冻结，促进相分离而无需移液，因此也提高了方法的精度。与需要通过移液除去有机层的传统萃取方法相比，开发的方法似乎省力省时。

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