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首页> 外文期刊>Brain research. Molecular brain research >Regional distribution of regulators of G-protein signaling (RGS) 1, 2, 13, 14, 16, and GAIP messenger ribonucleic acids by in situ hybridization in rat brain.
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Regional distribution of regulators of G-protein signaling (RGS) 1, 2, 13, 14, 16, and GAIP messenger ribonucleic acids by in situ hybridization in rat brain.

机译：在大鼠脑中通过原位杂交的G蛋白信号（RGS）1、2、13、14、16和GAIP信使核糖核酸调节剂的区域分布。

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摘要

Regulators of G-protein signaling (RGS) proteins are a novel family of GTPase-activating proteins that interact with Galpha subunits of the Gi/o, Gz, Gq and G(12/13) subfamilies to dampen G-protein-coupled receptor (GPCR)-mediated signaling by accelerating intrinsic Galpha-GTPase activity. In the present study, we report on messenger ribonucleic acid (mRNA) localization in rat brain of six RGS genes by in situ hybridization. The distribution patterns of RGS2, RGS13, RGS14 and GAIP (Galpha interacting protein) overlapped in most brain regions examined. Highest regional expression was observed for RGS2 in the cerebral cortical layers, striatum, hippocampal formation, several thalamic and hypothalamic nuclei and hindbrain regions such as the pontine, interpeduncular and dorsal raphe nuclei. Levels of RGS14 mRNA closely paralleled those of RGS2 expression levels throughout most brain regions. RGS13 mRNA was enriched in the hippocampal formation, amygdala, mammillary nuclei as well as the pontine and interpeduncular nuclei. GAIP expression levels were highest in the hippocampal formation with moderate to low levels present in all other regions studied. Of the six RGS genes probed, RGS16 mRNA displayed a discrete localization predominantly in the thalamic midline/intralaminar and principal relay nuclei, and the hypothalamic suprachiasmatic nucleus. RGS1 mRNA signal was not detected in brain. In conclusion, the in situ hybridization studies for RGS2, RGS13, RGS14, RGS16 and GAIP mRNAs extend our knowledge of the distribution of RGS genes expressed in the rat central nervous system, and indicate overlapping RGS-enriched regions that may be indicative of functional diversification in GPCR signaling pathway modulation.
机译：G蛋白信号转导（RGS）蛋白的调节剂是一种新的GTPase激活蛋白家族，可与Gi / o，Gz，Gq和G（12/13）亚家族的Galpha亚基相互作用，从而抑制G蛋白偶联受体（ GPCR）介导的信号传导，通过加速内在Galpha-GTPase活性。在本研究中，我们通过原位杂交报告了六个RGS基因在大鼠脑中的信使核糖核酸（mRNA）定位。 RGS2，RGS13，RGS14和GAIP（Galpha相互作用蛋白）的分布模式在大多数被检查的大脑区域中重叠。 RGS2在大脑皮层，纹状体，海马形成，几个丘脑和下丘脑核以及后脑区域（例如桥脑，椎间盘和背ra核）中观察到最高的区域表达。在大多数大脑区域中，RGS14 mRNA的水平与RGS2表达水平非常相似。 RGS13 mRNA富集于海马结构，杏仁核，乳头状核以及桥脑和足突间核。 GAIP表达水平在海马结构中最高，在所有其他研究区域中均存在中等至低水平。在探查的六个RGS基因中，RGS16 mRNA主要在丘脑中线/腹腔内和主要继发核以及下丘脑超视交叉核中表现出离散的定位。在大脑中未检测到RGS1 mRNA信号。总之，对RGS2，RGS13，RGS14，RGS16和GAIP mRNA的原位杂交研究扩展了我们对大鼠中枢神经系统表达的RGS基因分布的认识，并指出了重叠的富含RGS的区域，这可能表明功能多样化在GPCR信号通路中的调控。

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