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首页> 外文期刊>Brain research. Brain research protocols >In vivo microdialysis in the visual cortex of awake cat. II: sample analysis by microbore HPLC-electrochemical detection and capillary electrophoresis-laser-induced fluorescence detection.
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In vivo microdialysis in the visual cortex of awake cat. II: sample analysis by microbore HPLC-electrochemical detection and capillary electrophoresis-laser-induced fluorescence detection.

机译:清醒猫的视觉皮层体内微透析。 II:通过微孔HPLC-电化学检测和毛细管电泳-激光诱导的荧光检测进行样品分析。

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摘要

Sampling and monitoring release of excitatory and inhibitory amino acids in the striate cortex of mammals will provide important information for visual system research. Two microbore high performance liquid chromatography-electrochemical detection methods and a capillary electrophoresis-laser induced fluorescence detection were developed to determine the inhibitory amino acid, gamma-aminobutyric acid and the excitatory amino acids, glutamate and aspartate in microdialysates of cat striate cortex. In the liquid chromatography method, samples were derivatized using OPA-TBT. Ten microliters of derivatized product was injected onto the microbore column (100 x 1 mm i.d., C8) for quantitative analysis. Electrochemical detection was employed. In the capillary electrophoresis method, samples were derivatized using fluorescein isothiocyanate and separated in borate buffer within 15 min, then detected by a laser-induced fluorescence detector.
机译:采样和监测哺乳动物的纹状皮层中兴奋性和抑制性氨基酸的释放将为视觉系统研究提供重要信息。建立了两种微孔高效液相色谱-电化学检测方法和毛细管电泳-激光诱导荧光检测方法,用于测定猫纹状皮层微透析液中的抑制性氨基酸γ-氨基丁酸和兴奋性氨基酸谷氨酸和天冬氨酸。在液相色谱法中,使用OPA-TBT将样品衍生化。将十微升衍生产物注入到微孔柱(内径100 x 1毫米,C8)上进行定量分析。采用电化学检测。在毛细管电泳方法中,使用异硫氰酸荧光素衍生样品,并在硼酸盐缓冲液中分离15分钟,然后通过激光诱导的荧光检测器进行检测。

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