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首页> 外文期刊>Injury >Assessment of hepatic inflammation after spinal cord injury using intravital microscopy.
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Assessment of hepatic inflammation after spinal cord injury using intravital microscopy.

机译：使用活体显微镜评估脊髓损伤后的肝炎症。

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OBJECTIVES: The liver has been shown to play a particularly important role in the initiation and progression of the early systemic inflammatory response (SIR) to spinal cord injury (SCI). The purpose of this study was to determine the time course of leucocyte recruitment to the liver, and to determine the effect of injury severity on the magnitude of leucocyte recruitment and hepatic injury. METHODS: Rats were randomly assigned to one of the following groups: uninjured, sham-injured (laminectomy and no cord injury), cord compressed or cord transected. At 30 min and 90 min after SCI rats had the left lobe of their livers externalised and visualised using intravital video microscopy. RESULTS: Thirty minutes after injury the total number of leucocytes per post-sinusoidal venule was significantly increased after cord transection compared to that in uninjured and sham-injured rats (P<0.05). Of these leucocytes, significantly more were adherent to venule walls (P<0.05). At 90 min the total number of leucocytes per post-sinusoidal venule and the number of adherent and rolling leucocytes was significantly increased after cord transection and cord compression (P<0.05). DISCUSSION: This is the first study to use intravital microscopy to visualise systemic inflammation in the liver following SCI. We have demonstrated immediate leucocyte recruitment to the liver within 30 min after injury and have shown that systemic inflammation increases with time after injury and with severity of injury.
机译：目的：已显示肝脏在对脊髓损伤（SCI）的早期全身炎症反应（SIR）的发生和发展中起特别重要的作用。这项研究的目的是确定白细胞募集到肝脏的时间过程，并确定损伤严重程度对白细胞募集和肝损伤程度的影响。方法：将大鼠随机分为以下一组：未受伤，假受伤（椎板切除术且无脊髓损伤），脊髓受压或脊髓横断。在SCI后30分钟和90分钟，大鼠的左肝外部化并使用活体视频显微镜观察。结果：损伤后30分钟，与未受伤和假手术的大鼠相比，脊髓横断后每个正弦后小静脉的白细胞总数显着增加（P <0.05）。在这些白细胞中，有更多的粘附在小静脉壁上（P <0.05）。在90分钟时，脊髓横断和压迫后，每个正弦后小静脉的白细胞总数以及粘附和滚动白细胞的数量均显着增加（P <0.05）。讨论：这是第一项使用活体显微镜观察SCI后肝脏系统性炎症的研究。我们已经证明受伤后30分钟内白细胞会立即募集到肝脏，并显示出全身炎症随着受伤后的时间和严重程度的增加而增加。

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