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In vivo microdialysis in the visual cortex of awake cat. III: histological verification.

机译:清醒猫的视觉皮层体内微透析。三:组织学验证。

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In vivo microdialysis sampling extracellular excitatory and inhibitory amino acids from the striate cortex of mammals will provide important information for visual system research. To facilitate the interpretation of microdialysis results, this protocol critically examines: (1) the location of probe implantation in the visual cortex using Nissl staining; (2) the morphological changes after probe implantation by visualization of neurons containing glutamate; (3) the morphological changes after probe implantation by visualization of gliosis using glial fibrillary acidic protein (GFAP) immunocytochemistry; (4) the implantation of the probe in sensory-deprived versus non-deprived cortical regions by visualization of neurons containing c-Fos protein after limited retinal lesion. The histochemical and immunocytochemical methods of Glu, GFAP and c-Fos used are described.
机译:体内微透析从哺乳动物的纹状皮层中取样细胞外兴奋性和抑制性氨基酸将为视觉系统研究提供重要信息。为了便于解释微透析结果,该协议严格检查:(1)使用Nissl染色在视觉皮层中植入探针的位置; (2)通过可视化含有谷氨酸的神经元观察探针植入后的形态变化; (3)使用神经胶质原纤维酸性蛋白(GFAP)免疫细胞化学观察胶质细胞增生后探针植入后的形态变化; (4)通过可视化视网膜病变后含有c-Fos蛋白的神经元的可视化,将探针植入感觉缺失和非缺失皮质区域。描述了使用的Glu,GFAP和c-Fos的组织化学和免疫细胞化学方法。

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