• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain research. Brain research protocols >Methods for inducing neuronal loss in preweanling rats using intracerebroventricular infusion of kainic acid.
【24h】

Methods for inducing neuronal loss in preweanling rats using intracerebroventricular infusion of kainic acid.

机译：脑室脑内注入海藻酸的诱导断奶前大鼠神经元丢失的方法。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Excitotoxins, such as kainic acid (KA), have been shown to produce neuronal degeneration in the adult rat brain. While preweanling rats have been shown to be relatively resistant to the neurotoxicity of lower doses of KA, the presence of neuronal loss at higher doses (of KA) has only begun to be investigated in such animals. A reliable method of producing neuronal loss in preweanling rats is to administer nmol concentrations of KA via intracerebroventricular (i.c.v.) injections on postnatal day 7 (P7). Using a three-dimensional, non-biased cell counting technique, we have shown that neuronal loss is observed in the CA3 subfield of the hippocampal formation at P45 and P75. Further, immunohistochemical studies of markers for cell death may be useful to examine the types of cellular processes associated with such neuronal loss. Data from our own experiments suggest the activation of immediate-early genes in the neuronal loss produced by KA administration at P7. This developmental animal model of neuronal loss may be useful in studying neurodevelopmental disorders where the onset of symptoms or cognitive deficits is thought to follow an early developmental insult.
机译：兴奋性毒素，例如海藻酸（KA），已显示在成年大鼠大脑中产生神经元变性。虽然已经表明断奶前的大鼠对较低剂量的KA的神经毒性有相对的抵抗力，但仅在此类动物中才开始研究较高剂量（KA）的神经元缺失的存在。在断奶前大鼠中产生神经元丢失的可靠方法是在出生后第7天（P7）通过脑室内（i.c.v.）注射施用nmol浓度的KA。使用三维无偏细胞计数技术，我们已经显示在P45和P75处海马结构的CA3子域中观察到神经元丢失。此外，细胞死亡标记物的免疫组织化学研究对于检查与这种神经元丢失有关的细胞过程的类型可能是有用的。来自我们自己实验的数据表明，在P7进行KA给药所产生的神经元丢失中，早期基因的激活。这种神经元丧失的发育动物模型可能在研究神经发育障碍中有用，在神经发育障碍中，症状或认知缺陷的发作被认为是在早期发育损伤之后发生的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号