首页> 外文期刊>Brain research. Brain research protocols >Radiochemical nitric oxide synthase activity determination in rat brain with fast and accurate HPLC analysis.
【24h】

Radiochemical nitric oxide synthase activity determination in rat brain with fast and accurate HPLC analysis.

机译:快速,准确的HPLC分析测定大鼠脑中放射化学一氧化氮合酶的活性。

获取原文
获取原文并翻译 | 示例
           

摘要

In the central nervous system, NOS activity is involved in several physiological events, such as refinement of afferent connections in development, or linking cerebral blood flow to neural activity in adulthood, and also in many pathological events, such as cell death in brain ischemia and regulation of vasospasm in hemorrhage. Therefore, we studied NOS activity in the CNS. We describe a fast and accurate method in which we use HPLC analysis to identify and quantify citrulline eluted by ion-exchange chromatography, thus implementing the current method to evaluate NOS activity. This technique could be readily applied for NOS activity determination not only in brain, but also in all other tissues.
机译:在中枢神经系统中,NOS活性涉及多种生理事件,例如完善发育中的传入连接或将脑血流与成年后的神经活动联系起来,还涉及许多病理事件,例如脑缺血和死亡的细胞死亡。调节血管痉挛出血。因此,我们研究了CNS中的NOS活性。我们描述了一种快速准确的方法,其中我们使用HPLC分析来鉴定和定量通过离子交换色谱法洗脱的瓜氨酸,从而实施当前的方法来评估NOS活性。该技术不仅可以在大脑中而且可以在所有其他组织中轻松应用于NOS活性测定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号