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首页> 外文期刊>Brain research. Brain research protocols >Fluoro-Jade and silver methods: application to the neuropathology of scrapie, a transmissible spongiform encephalopathy.
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Fluoro-Jade and silver methods: application to the neuropathology of scrapie, a transmissible spongiform encephalopathy.

机译：荧光玉石和银的方法：应用于瘙痒病（一种可传播的海绵状脑病）的神经病理学。

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Traditional methods for evaluating neurodegeneration include variations of Nauta's selective silver-staining techniques. The Fluoro-Jade (FJ) method applies a novel fluorescent, anionic stain for localizing degenerating neurons. FJ has produced comparable results to the silver methods, when both have been applied to detect neurodegeneration in animals treated acutely with a variety of neurotoxins, including kainic acid (KA), ibogaine (IBO), 3-nitropropionic acid (3-NPA), domoic acid and others. The potential value of methods selective for neurodegeneration in elucidating the pathophysiology of transmissible spongiform encephalopathies (TSEs), such as the prion disease 'scrapie', has not yet been investigated. Using frozen or paraffin sections stained with FJ or silver, we evaluated the brains of hamsters inoculated with either the 263K or the 139H strains of scrapie, originally passaged from sheep into mice and then into hamsters. As a positive control, we also examined sections from IBO-treated rats, which experience degeneration restricted to small clusters of Purkinje neurons located in the paravermal region of the cerebellum. As expected, both FJ and silver methods delineated this identical pattern of neurodegeneration, characteristic of IBO exposure. Surprisingly, only a small number of FJ or silver-labeled cortical neurons were observed in scrapie-infected hamsters evaluated near the end of their incubation period but before obvious spongiform pathology. Instead, there was intense fluorescent staining of astrocytes in scrapie-infected hamsters, especially in the cortex, corpus callosum, and hypothalamus. Detailed protocols describing the application of the degeneration-selective methods we utilized are presented and compared.
机译：评估神经退行性变的传统方法包括Nauta的选择性银染技术的变化。 Fluoro-Jade（FJ）方法应用了一种新型的荧光，阴离子染色剂来定位退化的神经元。 FJ与白银方法相比，当两种方法都可用于检测用多种神经毒素（包括海藻酸（KA），伊博加因（IBO），3-硝基丙酸（3-NPA），海藻酸等。尚未研究选择性神经变性的方法在阐明可传播的海绵状脑病（TSE）的病理生理方面的潜在价值，例如TS病毒疾病“ sc”。我们使用FJ或银染色的冷冻或石蜡切片，评估了接种了263K或139H瘙痒病菌株的仓鼠的大脑，这些菌株最初是从绵羊传给小鼠，然后传给仓鼠的。作为阳性对照，我们还检查了接受IBO治疗的大鼠的切片，这些切片的变性仅限于位于小脑顶静脉区的小束Purkinje神经元。不出所料，FJ和银法都描述了这种相同的神经变性模式，即IBO暴露的特征。令人惊讶的是，在潜伏期接近结束但明显的海绵状病理之前，在被瘙痒病感染的仓鼠中仅观察到少量FJ或银标记的皮质神经元。相反，在瘙痒病感染的仓鼠中，尤其是在皮层，call体和下丘脑中，星形胶质细胞有强烈的荧光染色。介绍并比较了描述我们利用的变性选择方法的应用的详细协议。

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