Non-invasive assessment of blood-brain barrier (BBB) permeability using a gamma camera to detect 99technetium-gluceptate extravasation in rat brain.

Esposito P; Jacobson S; Connolly R; Gheorghe D; Theoharides TC

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Non-invasive assessment of blood-brain barrier (BBB) permeability using a gamma camera to detect 99technetium-gluceptate extravasation in rat brain.

机译：使用伽马相机对大鼠脑中99-葡萄糖酸盐外渗进行无创评估血脑屏障（BBB）渗透性。

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The blood-brain barrier (BBB) is a complex structure of endothelial cells, astroglia, pericytes, and perivascular macrophages enclosed by basal lamina. The BBB regulates the entry of blood-borne molecules and cells into the brain, but it is disrupted in various inflammatory conditions of the central nervous system (CNS). We previously showed that 30 min of immobilization stress increased 99technetium-gluceptate (99Tc) extravasation, measured by a gamma counter, in brain regions containing mast cells, an effect blocked by the mast cell stabilizer disodium cromoglycate [Brain Res. 888 (2001) 117]. Here we report the use of a gamma camera to assess BBB permeability by assessing 99Tc extravasation in the rat brain, during and following acute stress, without having to sacrifice the experimental animals. This method also allows for repeated experimentation on the same animal, since the half-life of 99Tc is only 6 h, and permits testing of potential inhibitors of BBB permeability.

机译：血脑屏障（BBB）是由基底层包围的内皮细胞，星形胶质细胞，周细胞和血管周巨噬细胞的复杂结构。 BBB调节血液传播的分子和细胞进入大脑，但在中枢神经系统（CNS）的各种炎症条件下会被破坏。我们以前显示固定的应力30分钟会在包含肥大细胞的大脑区域中增加99葡糖酸铵（99Tc）的渗出（通过伽玛计数器测量），这种作用被肥大细胞稳定剂色甘酸二钠[Brain Res。 888（2001）117]。在这里，我们报告了使用伽玛相机通过评估急性应激期间和之后大鼠脑内99Tc外渗来评估BBB渗透性，而不必牺牲实验动物。该方法还允许对同一动物进行重复实验，因为99Tc的半衰期仅为6小时，并允许测试BBB通透性的潜在抑制剂。

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《Brain research. Brain research protocols》 |2001年第2期|共7页
作者
Esposito P; Jacobson S; Connolly R; Gheorghe D; Theoharides TC;
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正文语种 eng
中图分类内科学;
关键词
Blood-Brain Barrier; Capillary Permeability; Cerebrovascular Circulation; 血脑屏障; 毛细血管通透性; 脑血管循环; 放射性核素显像;

机译：Blood-Brain Barrier;Capillary Permeability;Cerebrovascular Circulation;血脑屏障;毛细血管通透性;脑血管循环;放射性核素显像;

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1. Non-invasive assessment of blood-brain barrier (BBB) permeability using a gamma camera to detect 99technetium-gluceptate extravasation in rat brain. [J] . Esposito P, Jacobson S, Connolly R, Brain research. Brain research protocols . 2001,第2期

机译：使用伽马相机对大鼠脑中99-葡萄糖酸盐外渗进行无创评估血脑屏障（BBB）渗透性。
2. The effect of blood-brain barrier (BBB)-specific laminins for barrier function with a new in vitro BBB model incorporating multi-culturing system of BBB components [J] . Y. Takeshita, S. Fujikawa, M. Omoto, Journal of the Neurological Sciences: Official Bulletin of the World Federation of Neurology . 2017,第期

机译：血型脑屏障（BBB）特异性层压族屏障功能的影响，具有掺入BBB组分多培养系统的新型体外BBB模型
3. Super Antibiotics, Part II. Hyperforin, Mass Spectroscopy (MS) and Gas Chromatography-Mass Spectrometry (GC-MS), Evidence of Permeability of the Blood-Testis Barrier (BTB) and the Blood-Brain Barrier (BBB) to Hyperforin [J] . Ilia Brondz International Journal of Analytical Mass Spectrometry and Chromatography . 2016,第4期

机译：超级抗生素，第二部分。 Hyperforin，质谱（MS）和气相色谱-质谱（GC-MS），血睾丸屏障（BTB）和血脑屏障（BBB）对Hyperforin的渗透性证据
4. Feasibility of Ghrelin1-9 as A Targeting Ligand across Blood-Brain Barrier: In VitroCellular Binding and BBB Permeability Studies [C] . Ya-Chin Lo, Yi-Ju Ko, Hsiang-Fa Liang, Annual meeting exposition of the Controlled Release Society . 2009

机译：Ghrelin 1-9作为跨血脑屏障的靶向配体的可行性：体外细胞结合和BBB渗透性研究
5. Examining the integrity of the blood-brain barrier (BBB) and the use of lysophosphatidic acid (LPA) to modulate the barrier properties. [D] . On, Ngoc H. 2012

机译：检查血脑屏障（BBB）的完整性以及使用溶血磷脂酸（LPA）调节屏障的性质。
6. Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport [O] . Yves Molino, Françoise Jabès, Emmanuelle Lacassagne, 2014

机译：建立大鼠血脑屏障（BBB）的体外模型：专注于血脑屏障的抗渗透性和受体介导的运输。
7. Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport [O] . Yves Molino, Françoise Jabès, Emmanuelle Lacassagne, 2014

机译：在体外设置A型血脑屏障模型（BBB）：对BBB不渗透性和受体介导的运输的关注
8. Combined Exposure to Pyridostigmine Bromide (PB), DEET, and Permethrin with Stress Increases Blood-Brain Barrier (BBB) Permeability and Inhibits Brain Acetycholinesterase in Rats. [R] . Abou-Donia, M. B., Khan, W. A., Suliman, H. B., 2001

机译：联合暴露于吡啶斯的明溴化物（pB），DEET和氯菊酯与应激增加血脑屏障（BBB）渗透性和抑制大鼠脑乙酰胆碱酯酶。

Non-invasive assessment of blood-brain barrier (BBB) permeability using a gamma camera to detect 99technetium-gluceptate extravasation in rat brain.

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