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A device for stereotactic transection of fiber bundles in rats.

机译：用于大鼠中纤维束的立体定向横切的装置。

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摘要

We have designed a device for stereotactic transection of fiber bundles in experiments using rats. Here, we present our assessment of its features relative to those of conventional methods. The instrument consists of a stainless steel cannula and a thin inner wire with a hook at one end and a hilt at the other. The hook can be extended or withdrawn freely by pushing or pulling the hilt. Lesions were evaluated in 12 male Wistar rats, after two targets, the anterior commissure (n=6) and the caudate-putamen (n=6), were transected. After the cannula was introduced into the target, the inner hook was extended in an anterior direction orthogonally to the transverse plane. Next, the entire device was pulled back along the insertion path to transect the neuronal fibers. Then the inner hook was withdrawn into the cannula and the entire device was removed. Seven days later, brains were removed for histologic processing. Microscopic examination demonstrated a slit like gap produced by transection at the target; the lesions were infiltrated by microglia and surrounded by gliosis. Adjacent regions were minimally damaged. The path of the cannula demonstrated only minimal gliosis. Unlike conventional methods, this device permits precise transection of deep fiber bundles with minimal damage to surrounding brain tissue.
机译：我们设计了一种在使用大鼠的实验中用于纤维束的立体定向横切的装置。在这里，我们介绍其相对于常规方法的功能评估。该仪器由一个不锈钢套管和一根细细的内线组成，一端有一个钩子，另一端有一个柄。可以通过推动或拉动柄来自由地延长或撤回挂钩。在将十二指肠前连合（n = 6）和尾状丘脑（n = 6）两个靶标切开后，在12只雄性Wistar大鼠中评估了病变。在将插管引入靶中之后，将内部钩沿与横向平面正交的向前方向延伸。接下来，将整个设备沿插入路径拉回以横切神经元纤维。然后将内部钩子抽回套管中，并移走整个装置。七天后，取出大脑进行组织学处理。显微镜检查显示在目标处横切产生狭缝状间隙。病变被小胶质细胞浸润并被神经胶质化所包围。相邻区域受到的损坏最小。套管的路径仅显示出极小的胶质增生。与传统方法不同，此设备可对深层纤维束进行精确横切，而对周围脑组织的损害却最小。

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