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Stimulation of dental pulp with simultaneous recording of the jaw opening reflex in the rat.

机译：刺激牙髓并同时记录大鼠颌骨开口反射。

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The processing of nociceptive information in the central nervous system has been analysed in most studies by activation of peripheral nerves. However, the limitation of this method is the simultaneous activation of noxious and inocuous fibers. Nevertheless, the stimulation of the tooth pulp is believed to activate mainly nociceptive fibers which could be the method of choice. On the other hand, the response to nociceptive activation of the dental pulp is easily quantified by the amplitude of the jaw opening reflex, a nocifensive flexion withdrawal reflex. In this protocol we describe a technique to manufacture and implant electrodes in lower incisors of the rat and a method to prepare and insert stainless steel twisted bipolar electrodes to record the electromyographic activity of both digastric muscles, in response to nociceptive dental pulp stimulation. This approach was applied in the study of the analgesic effects of the rat's striatal stimulation.
机译：在大多数研究中，通过激活周围神经来分析中枢神经系统中伤害感受信息的处理。但是，该方法的局限性是有毒和无害纤维的同时活化。然而，据信刺激牙髓主要激活伤害性纤维，这可以作为选择的方法。另一方面，通过下颌张开反射，伤害性屈曲撤回反射的幅度容易地量化对牙髓的伤害性激活的响应。在该协议中，我们描述了一种在大鼠下门牙中制造和植入电极的技术，以及一种制备和插入不锈钢扭曲双极电极以记录对两条牙龈肌肉的伤害性牙髓刺激的肌电活动的方法。该方法被用于研究大鼠纹状体刺激的镇痛作用。

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