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Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays

机译:微流控技术与细胞生物学相遇:通过验证和应用微尺度技术进行细胞生物学测定来弥合差距

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摘要

Microscale techniques have been applied to biological assays for nearly two decades, but haven't been widely integrated as common tools in biological laboratories. The significant differences between several physical phenomena at the microscale versus the macroscale have been exploited to provide a variety of new types of assays (such as gradient production or spatial cell patterning). However, the use of these devices by biologists seems to be limited by issues regarding biological validation, ease of use, and the limited available readouts for assays done using microtechnology. Critical validation work has been done recently that highlights the current challenges for microfluidic methods and suggest ways in which future devices might be improved to better integrate with biological assays. With more validation and improved designs, microscale techniques hold immense promise as a platform to study aspects of cell biology that are not possible using current macroscale techniques. BioEssays 30:811-821, 2008.
机译:微型技术已用于生物测定已有近二十年的历史,但尚未广泛集成为生物实验室中的常用工具。在微观尺度上与宏观尺度上的几种物理现象之间的显着差异已经被利用来提供各种新型的检测方法(例如梯度产生或空间细胞图案化)。但是,生物学家对这些设备的使用似乎受到以下方面的限制:生物学验证,易用性以及使用微技术完成的测定方法的可用读数有限。最近已经进行了关键的验证工作,突出了微流体方法当前面临的挑战,并提出了改进未来设备以更好地与生物学测定结合的方法。通过更多的验证和改进的设计,微尺度技术有望成为研究细胞生物学各方面的平台,而这是当前大尺度技术无法实现的。 BioEssays 30:811-821,2008。

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