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首页> 外文期刊>european journal of phycology >#x3B1;-1,4-Glucan lyase-producing endophyte ofGracilariopsissp. (Rhodophyta) from China
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#x3B1;-1,4-Glucan lyase-producing endophyte ofGracilariopsissp. (Rhodophyta) from China

机译：α-1,4-葡聚糖裂解酶生产内生菌Gracilariopsissp.（Rhodophyta）来自中国

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Purification of #x3B1;-1,4-glucan lyase from red algae and fungi has previously been reported. The #x3B1;-glucan lyase converts #x3B1;-glucans to 1,5- anhydro-d-fructose. In this study, an endophyte was detected between the algal cells ofGracilariopsissp., but not penetrating the cell walls. Histological staining was consistent with the endophyte being fungal and immunohistochemistry revealed that it possessed an #x3B1;-1,4-glucan lyase differing from that of the host. The endophyte #x3B1;-glucan lyase was recognized by antisera against both fungal and red algal #x3B1;-glucan lyases, whereas the #x3B1;-glucan lyase found in the algal tissue was recognized only by the antisera against algal #x3B1;-glucan lyase. The lyases occur independently in the host and in the endophyte, and were sometimes detected simultaneously. The endophyte was only observed inGracilariopsissp. collected in China; no endophyte could be detected inGracilariopsissp. from California or inGracilaria chilensis.
机译：以前曾报道过从红藻和真菌中纯化 α-1,4-葡聚糖裂解酶。α-葡聚糖裂解酶将α-葡聚糖转化为1,5-脱水-D-果糖。在这项研究中，在Gracilariopsissp.的藻类细胞之间检测到内生菌，但没有穿透细胞壁。组织学染色与内生菌为真菌一致，免疫组化显示其具有与宿主不同的α-1,4-葡聚糖裂解酶。内生菌 α-葡聚糖裂解酶被抗真菌和红藻 α-葡聚糖裂解酶的抗血清识别，而藻类组织中发现的 α-葡聚糖裂解酶仅被抗藻 α-葡聚糖裂解酶的抗血清识别。裂解酶在宿主和内生菌中独立发生，有时同时被检测到。内生菌仅在Gracilariopsissp中观察到。在中国收集;在Gracilariopsissp中未检测到内生菌。来自加利福尼亚或 inGracilaria chilensis。

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