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首页> 外文期刊>Innate immunity >Alterations in Helicobacter pylori outer membrane and outer membrane vesicle-associated lipopolysaccharides under iron-limiting growth conditions
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Alterations in Helicobacter pylori outer membrane and outer membrane vesicle-associated lipopolysaccharides under iron-limiting growth conditions

机译：限铁生长条件下幽门螺杆菌外膜和外膜囊泡相关脂多糖的变化

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摘要

Outer membrane vesicles (OMVs) shed from the gastroduodenal pathogen Helicobacter pylori have measurable effects on epithelial cell responses. The aim of this study was to determine the effect of iron availability, and its basis, on the extent and nature of lipopolysaccharide (LPS) produced on H. pylori OMVs and their parental bacterial cells. Electrophoretic, immunoblotting and structural analyses revealed that LPSs of bacterial cells grown under iron-limited conditions were notably shorter than those of bacteria and OMVs obtained from iron-replete conditions. Structural analysis and serological probing showed that LPSs of iron-replete cells and OMVs expressed O-chains of Lewis x with a terminal Lewis y unit, whereas Lewis y expression was notably reduced on bacteria and OMVs from iron-limiting conditions. Unlike the O-chain, the core oligosaccharide and lipid A moieties of iron-replete and iron-limited bacteria and their OMVs were similar. Quantitatively, shed OMVs from iron-replete bacteria were found to be LPS enriched, whereas shed OMVs from iron-limited bacteria had a significantly reduced content of LPS. These differences were linked to bacterial ATP levels. Since iron availability affects the extent and nature of LPS expressed by H. pylori, host iron status may contribute to H. pylori pathogenesis.
机译：从胃十二指肠病原体幽门螺杆菌脱落的外膜囊泡（OMV）对上皮细胞反应具有可测量的作用。这项研究的目的是确定铁的有效性及其基础，取决于幽门螺杆菌OMV及其亲代细菌细胞产生的脂多糖（LPS）的程度和性质。电泳，免疫印迹和结构分析表明，在铁受限条件下生长的细菌细胞的LPS明显短于从铁充足条件下获得的细菌和OMV的LPS。结构分析和血清学探测表明，铁含量高的细胞和OMV的LPS表达带有末端Lewis y单位的Lewis x的O链，而铁限制条件下细菌和OMV的Lewis y表达则明显降低。与O链不同，富铁和铁受限细菌的核心寡糖和脂质A部分及其OMV相似。从数量上看，发现来自铁含量丰富的细菌的脱落OMV富含LPS，而来自铁含量有限的细菌的脱落OMV的LPS含量明显降低。这些差异与细菌ATP水平有关。由于铁的可用性影响幽门螺杆菌表达的LPS的程度和性质，因此宿主铁的状态可能有助于幽门螺杆菌的发病。

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