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Molecular diversity study on dessert banana genotypes (Musa spp.) from Odisha using ISSR markers

机译:利用ISSR标记研究奥里萨邦甜香蕉基因型(Musa spp。)的分子多样性

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Banana is an important fruit of India and in Odisha it is also a highly demanded fruit. Banana improvement programme mainly depends on genetic variability present in the population. The present investigation was done to assess the molecular diversitypresent amongst the local dessert banana genotypes of Odisha along with some national released cultivars based on Inter Simple Sequence Repeats (ISSR) markers. For the present study ten ISSR primers were used to differentiate 22 banana genotypes. Total seventy six scorable fragments were obtained, out of which 36 (47.4%) were polymorphic and 39 (51.3%) were monomorphic. Similarity index were estimated using the Dice coefficient of similarity (Nei and Li 1979). The genetic similarity values ranged from 0.71 to 0.96. At 90 % phenon level 22 genotypes were distributed in fourteen clusters. The results revealed twenty five percent variability at genetic level based on ten ISSR markers which could be utilised for further banana improvement programme.
机译:香蕉是印度的重要水果,在奥里萨邦,它也是需求量很大的水果。香蕉改良计划主要取决于人口中存在的遗传变异性。本研究的目的是评估基于间作简单重复序列(ISSR)标记的Odisha本地甜点香蕉基因型以及一些国家发布的品种之间的分子多样性。对于本研究,使用十个ISSR引物来区分22种香蕉基因型。共获得76个可评分片段,其中36个(47.4%)是多态性的,39个(51.3%)是单态性的。相似度指数是使用Dice相似度系数估算的(Nei和Li,1979)。遗传相似性值介于0.71至0.96之间。在90%的phenon水平上,22个基因型分布在14个簇中。结果显示,基于十个ISSR标记,在遗传水平上有25%的变异性,可用于进一步的香蕉改良计划。

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