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首页> 外文期刊>International Biodeterioration & Biodegradation >Cr(VI) reduction in naturally rich growth medium and sugarcane bagasse by Acinetobacter haemolyticus.
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Cr(VI) reduction in naturally rich growth medium and sugarcane bagasse by Acinetobacter haemolyticus.

机译：溶血不动杆菌可自然富集生长培养基和甘蔗渣中六价铬（VI）的减少。

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摘要

The potential of agricultural waste as substitute for rich-growth medium for bacteria during Cr(VI) reduction was investigated using the locally isolated Cr(VI) resistant-reducing strain of Acinetobacter haemolyticus and sugarcane bagasse. A. haemolyticus showed higher resistance to 100 mg/L Cr(VI) in Luria Bertani (>1010 CFU/mL) compared to sugarcane bagasse (106 CFU/mL). Higher Cr(VI) reduction by the SCB-immobilized bacteria (>90%) compared to in LB only (around 25%) clearly indicated the role of SCB in carrying out abiotic reduction of Cr(VI) as well as Cr(VI) adsorption. Nevertheless, Cr(III) was detected on the bacterial surface using Electron Spin Resonance (ESR) and electron microscopy (FESEM-EDX) analysis. The Cr(III) deposition occurred probably via complex formation with either carboxyl, hydroxyl or amide groups present on the bacterial cells surface as suggested from the FT-IR analysis. TEM analysis further showed Cr distribution at the membrane and cytosolic fractions. This work clearly demonstrated the role of bacteria in reducing Cr(VI) to Cr(III) as well as the potential of using agricultural waste material such as SCB to carry out abiotic Cr(VI) reduction.
机译：使用局部分离的溶血不动杆菌和甘蔗渣研究了农业废料在还原六价铬过程中作为细菌丰富生长培养基的潜力。与甘蔗渣（10 6 CFU / mL）相比，溶血曲霉对Luria Bertani（> 10 10 CFU / mL）的100 mg / L Cr（VI）表现出更高的抗性）。与仅在LB中（约25％）相比，固定有SCB的细菌对Cr（VI）的还原率更高（> 90％），这清楚地表明了SCB在非生物还原Cr（VI）和Cr（VI）中的作用吸附。然而，使用电子自旋共振（ESR）和电子显微镜（FESEM-EDX）分析在细菌表面检测到了Cr（III）。 FT-IR分析表明，Cr（III）的沉积可能是通过与细菌细胞表面上存在的羧基，羟基或酰胺基形成络合物而发生的。 TEM分析进一步显示Cr在膜和胞质部分的分布。这项工作清楚地证明了细菌在将Cr（VI）还原为Cr（III）中的作用，以及使用农业废料（例如SCB）进行非生物Cr（VI）还原的潜力。

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