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首页> 外文期刊>International Biodeterioration & Biodegradation >Contrasting characteristics of anthracene and pyrene degradation by wood rot fungus Pycnoporus sanguineus H1
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Contrasting characteristics of anthracene and pyrene degradation by wood rot fungus Pycnoporus sanguineus H1

机译：木腐真菌Pycnoporus sanguineus H1降解蒽和pyr的对比特征

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摘要

This investigation evaluates the contrasting metabolic characteristics of anthracene and pyrene by wood rot fungus Pycnoporus sanguineus H1. Under in vivo conditions, P. sanguineus H1 degraded 67.5% of the anthracene and showed maximal laccase activity of 1568.2 U L-1. When piperonyl butoxide (PB) was added to the liquid cultures, the anthracene degradation rate increased to 73.1% and maximal laccase activity increased to 2034.3 U L-1. For pyrene, the degradation rate was 31.1%; however, after PB addition, the value decreased to 5.3% while maximal laccase activity increased to 1625.3 U L-1 from 1469.1 U L-1. Under in vitro conditions, the extracellular liquid culture (10 kDa membrane, ultra-filtered) transformed 59.9% of the anthracene, while addition of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) enhanced the anthracene transformation rate up to 92.0%. The extracellular liquid did not convert the pyrene, however, suggesting that anthracene is modified extracellularly, (likely via laccase) while pyrene is not. Whole mycelium, as well as homogenized mycelium and microsomal proteins, transformed both anthracene and pyrene; conversion was inhibited to some extent by the PB, implying that cytochrome P450 participates in intracellular PAHs transformation. Additional evidence that ABTS enhances anthracene transformation by homogenized mycelium suggests that mycelium-associated laccase cooperates in the intracellular degradation of anthracene. These results altogether elucidate the metabolism of PAHs by P. sanguineus via extracellular laccase, intracellular cytochrome P450, and mycelium-associated laccase. (C) 2015 Elsevier Ltd. All rights reserved.
机译：该研究评估了木腐真菌碧萝yH1对蒽和pyr的代谢特性的差异。在体内条件下，血红球菌H1降解了蒽的67.5％，并显示出最大的漆酶活性为1568.2 U L-1。当将胡椒基丁醚（PB）添加到液体培养物中时，蒽的降解率增加到73.1％，最大的漆酶活性增加到2034.3 U L-1。 pyr的降解率为31.1％。然而，添加PB后，该值降低至5.3％，而最大漆酶活性从1469.1 U L-1增加至1625.3 U L-1。在体外条件下，细胞外液体培养物（10 kDa膜，超滤）转化了59.9％的蒽，而添加2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）增强了蒽的转化率高达92.0％。然而，细胞外液并未转化the，这表明蒽在细胞外被修饰（可能通过漆酶），而pyr未被修饰。整个菌丝体以及均质的菌丝体和微粒体蛋白都转化了蒽和pyr。 PB在某种程度上抑制了细胞的转化，这表明细胞色素P450参与了细胞内PAHs的转化。 ABTS通过均质的菌丝体增强蒽转化的其他证据表明，与菌丝体相关的漆酶可协同蒽的胞内降解。这些结果完全阐明了血红假单胞菌通过细胞外漆酶，细胞内细胞色素P450和菌丝体相关漆酶对PAHs的代谢。 （C）2015 Elsevier Ltd.保留所有权利。

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