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Comparative proteomic analysis of phenol degradation process by Arthrobacter

机译：节杆菌对苯酚降解过程的比较蛋白质组学分析

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摘要

Phenol is a widespread environmental pollutant due to their broad usage and applications, and presence in many industrial effluents. To date, bacterial degradation of phenol remains to be the preferred method for its removal and remediation. Although the degradation pathway has been extensively studied, the variations in the level of expression of the key enzymes during catabolism are still not quantitatively understood. An explicit quantitative expression helps us know the degradation process in detail. It is important and valuable for us to further understand mechanisms underlying phenol degradation. In this study, we used iTRAQ-based comparative proteomics analysis approach to determine variations in expression and regulation of key enzymes in Arthrobacter during phenol degradation. We propose that the phenol biodegradation pathway is mainly determined by 5 pivotal enzymes, which belonged to 3-oxoadipate pathway and tricarboxylic acid cycle. Arthrobacter mainly degrades phenol through 3-oxoadipate pathway, which makes the pathway of this strain for degrading the toxic compound clearer. These findings provide new insights into phenol biodegradation process and would help us understand the step by step stages of this metabolic pathway. (C) 2016 Elsevier Ltd. All rights reserved.
机译：苯酚由于其广泛的用途和应用以及在许多工业废水中的存在而成为一种广泛的环境污染物。迄今为止，苯酚的细菌降解仍然是去除和修复苯酚的首选方法。尽管已经对降解途径进行了广泛的研究，但分解代谢过程中关键酶表达水平的变化仍未得到定量了解。明确的定量表达有助于我们详细了解降解过程。对我们来说，进一步了解苯酚降解的机理很重要，也很有价值。在这项研究中，我们使用了基于iTRAQ的比较蛋白质组学分析方法来确定在苯酚降解过程中节杆菌中关键酶的表达和调控的变化。我们认为，苯酚的生物降解途径主要由5种关键酶决定，分别属于3-氧己二酸途径和三羧酸循环。节杆菌主要通过3-氧代己二酸途径降解苯酚，这使该菌株降解有毒化合物的途径更加清晰。这些发现提供了有关苯酚生物降解过程的新见解，将有助于我们逐步了解该代谢途径。 （C）2016 Elsevier Ltd.保留所有权利。

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