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首页> 外文期刊>International Biodeterioration & Biodegradation >Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs
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Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs

机译：细菌bamA基因的分子多样性参与中温石油储层芳烃的厌氧降解。

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摘要

Aromatic hydrocarbons are usually metabolized to the central intermediate benzoyl-coenzyme A (CoA) prior to ring cleavage. 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, a key enzyme involved in the reductive benzene ring cleavage, encoded by the bamA gene can serve as a functional biomarker for detecting anaerobic degradation of aromatic compounds in different environments. In the present study, we examined the diversity the bacterial bamA gene in oil field. We sequenced bamA fragments that were PCR amplified from the microbial biomass of two mesophilic oil producing reservoirs during a period of four months. The results showed the presence and a higher diversity of bamA gene than previous studies, indicating its potential involvement in degrading aromatic hydrocarbons through anaerobic biochemical pathways in oil fields. The bamA gene sequences were affiliated to bacteria that are known to degrade aromatics, including Azoarcus, Georgfuchsia, Sulfuritalea, Peptococcaceae and Syntrophus. Differences of composition and diversity of bamA gene sequences over a period of four months in these oil reservoirs showed that it is necessary to monitor functional genes over a period of time to fully establish their significance to any specific process at any site. (C) 2016 Elsevier Ltd. All rights reserved.
机译：芳烃通常在环裂解之前被代谢成中间中间体苯甲酰基辅酶A（CoA）。 bamA基因编码的6-氧代环己-1-烯-1-羰基-CoA水解酶是参与还原性苯环裂解的关键酶，可作为功能性生物标志物，用于检测不同环境中芳香族化合物的厌氧降解。在本研究中，我们研究了油田中细菌bamA基因的多样性。我们对bamA片段进行了测序，这些片段在两个月的时间内从两个中温产油油藏的微生物生物质中进行了PCR扩增。结果表明bamA基因的存在和多样性比以前的研究更高，表明它可能通过厌氧生化途径在油田中降解芳香烃。 bamA基因序列与已知能降解芳香族化合物的细菌有关，包括固氮菌，Georgfuchsia，Sulfuritalea，Peptococcaceae和Syntrophus。在这些油藏中，四个月内bamA基因序列的组成和多样性的差异表明，有必要在一段时间内监控功能基因，以完全确立其对任何地点任何特定过程的重要性。 （C）2016 Elsevier Ltd.保留所有权利。

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