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首页> 外文期刊>International immunopharmacology >An anti-inflammatory effect of murine fetal liver cells in BALB/c mouse contact hypersensitivity model.
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An anti-inflammatory effect of murine fetal liver cells in BALB/c mouse contact hypersensitivity model.

机译:鼠胎儿肝细胞在BALB / c小鼠超敏反应模型中的抗炎作用。

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摘要

Anti-inflammatory effects of murine fetal liver (FL) cells were studied using BALB/c mouse contact hypersensitivity (paw edema) model. Paw weight differences, lymphatic organ weights, hematological and histological indices as well as proinflammatory (TNF-alpha) and anti-inflammatory (IL-10) cytokine levels in sera were evaluated. Immunophenotyping revealed that both murine FL homogenate cells (HC) and FL hematopoietic stem cells (HSC) express CD117 and CD38 surface markers. Single doses of 1x10(6) cells/mouse and 2x10(6) cells/mouse of FL HC as well as of FL HSC, when used separately, all statistically significantly (p<0.05) inhibited paw edema, but the lower dose was more effective and giving results similar to that of prednisolone. Either dose of FL HC or FL HSC studied had no significant influence on lymphatic organ weights; no significant changes were also observed in blood indices. The data of cytokine studies showed that TNF-alpha concentration in sera of mice treated with either FL HC or FL HSC at a dose of 1x10(6) cells/mouse was statistically significantly (p<0.001) lower than that of the control mice. A concentration of IL-10 was statistically significantly higher (p<0.01) in mice treated with a dose of 1x10(6) cells/mouse of FL HC but not with the same dose of FL HSC as compared to the control group. Histological examination revealed better effects of a dose of 1x10(6) cells/mouse of FL HC when compared with the same dose of FL HSC as in regard to reduction of edema thickness and cell infiltration.
机译:使用BALB / c小鼠接触超敏反应(爪水肿)模型研究了鼠胎肝(FL)细胞的抗炎作用。评估了足爪重量差异,淋巴器官重量,血液学和组织学指数以及血清中促炎性(TNF-alpha)和抗炎性(IL-10)细胞因子的水平。免疫分型显示,小鼠FL匀浆细胞(HC)和FL造血干细胞(HSC)均表达CD117和CD38表面标记。单独使用FL HC和FL HSC的1x10(6)个细胞/小鼠和2x10(6)个细胞/小鼠,单独使用时,统计学上均具有显着(p <0.05)抑制爪水肿的作用,但较低剂量的作用更大有效,并给出与泼尼松龙相似的结果。所研究的FL HC或FL HSC剂量对淋巴器官重量均无显着影响。血液指标也没有观察到显着变化。细胞因子研究的数据表明,以1x10(6)个细胞/小鼠的剂量用FL HC或FL HSC治疗的小鼠血清中的TNF-α浓度在统计学上显着低于对照小鼠(p <0.001)。与对照组相比,在用1x10(6)细胞/小鼠FL HC处理但未用相同剂量的FL HSC处理的小鼠中,IL-10的浓度在统计学上显着较高(p <0.01)。组织学检查显示,与减少同等剂量的FL HSC相比,就减少水肿厚度和细胞浸润而言,每只FL HC小鼠1x10(6)个细胞剂量的效果更好。

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