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首页> 外文期刊>International immunopharmacology >Lonchocarpus sericeus lectin decreases leukocyte migration and mechanical hypernociception by inhibiting cytokine and chemokines production.
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Lonchocarpus sericeus lectin decreases leukocyte migration and mechanical hypernociception by inhibiting cytokine and chemokines production.

机译：扁果沙棘凝集素通过抑制细胞因子和趋化因子的产生来减少白细胞迁移和机械性神经痛。

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In this study, we tested the potential use of a lectin from Lonchocarpus sericeus seeds (LSL), to control neutrophil migration and inflammatory hypernociception (decrease of nociceptive threshold). Pretreatment of the animals intravenously (15 min before) with LSL inhibited neutrophil migration to the peritoneal cavity in a dose-dependent fashion confirmed by an inhibition of rolling and adhesion of leukocytes by intravital microscopy. We also tested the ability of the pretreatment with LSL to inhibit neutrophil migration on immunised mice, and it was observed that a strong inhibition of neutrophil migration induced by ovoalbumin in immunized mice. Another set of experiments showed that pretreatment of the animals with LSL, inhibited the mechanical hypernociception in mice induced by the i.pl. injection of OVA in immunized mice and of carrageenan in naive mice, but not that induced by prostaglandin E(2) (PGE(2)) or formalin. This anti-nociceptive effect correlated with an effective blockade of neutrophil influx, as assessed by the hind paw tissue myeloperoxidase levels. In addition, we measured cytokines (TNF-alpha and IL-1beta) and chemokines (MIP-1alpha [CCL3] and KC [CXCL1]) from the peritoneal exudates and i.pl. tissue. Animals treated with LSL showed inhibition of cytokines and chemokines release in a dose-dependent manner. In conclusion, we demonstrated that the inhibitory effects of LSL on neutrophil migration and mechanical inflammatory hypernocicepetion are associated with the inhibition of the production of cytokines and chemokines.
机译：在这项研究中，我们测试了潜在的使用丝裂藻种子（LSL）中的凝集素来控制中性粒细胞迁移和炎性痛觉过敏（伤害感受性阈值的降低）。用LSL对动物进行静脉预处理（15分钟前）以剂量依赖性方式抑制嗜中性白细胞向腹膜腔的迁移，这通过活体显微镜检查抑制了白细胞的滚动和粘附得以证实。我们还测试了用LSL预处理抑制免疫小鼠中嗜中性粒细胞迁移的能力，并且观察到卵清蛋白诱导的免疫小鼠中性粒细胞迁移具有很强的抑制作用。另一组实验表明，用LSL预处理动物可抑制i.pl诱导的小鼠机械性神经痛。在免疫小鼠中注射OVA，在幼稚小鼠中注射角叉菜胶，但不注射前列腺素E（2）（PGE（2））或福尔马林诱导的剂量。如通过后爪组织髓过氧化物酶水平评估的，这种抗伤害感受的作用与中性粒细胞流入的有效阻断有关。另外，我们从腹膜渗出液和i.pl中测量了细胞因子（TNF-α和IL-1β）和趋化因子（MIP-1α[CCL3]和KC [CXCL1]）。组织。用LSL治疗的动物表现出剂量依赖性抑制细胞因子和趋化因子的释放。总之，我们证明了LSL对嗜中性粒细胞迁移和机械性炎症反应过度的抑制作用与抑制细胞因子和趋化因子的产生有关。

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