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首页> 外文期刊>International immunopharmacology >Anti-inflammatory activity of phlomisoside F isolated from Phlomis younghusbandii Mukerjee
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Anti-inflammatory activity of phlomisoside F isolated from Phlomis younghusbandii Mukerjee

机译：芦荟中芦荟苷F的抗炎活性

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This study was designed to investigate the anti-inflammatory effect of phlomisoside F (PMF) isolated from Phlomis younghusbandii and to explore the possible pharmacological mechanisms. Anti-inflammatory effects of PMF were evaluated by using carrageenan-induced rat paw edema test, dimethylbenzen-induced ear edema test, acetic acid-induced vascular permeability and cotton pellet granuloma test. Furthermore, the releases of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) were determined by ELISA. To explore the potential mechanisms, expressions of iNOS and COX-2 were determined by quantitative real-time PCR and western blotting assays. In addition, the expressions of nuclear p65, cytosolic p65, I kappa B, p38, p-p38, p-ERK1/2, ERK1/2, JNK and p-JNK were determined by western blotting assay. Our results indicated that PMF administered orally could not only significantly decrease rat paw edema in rats and ear edema in mice, but also reduce the vascular permeability in mice and granuloma weights in rats. In vitro, the releases of LPS-induced pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1 beta) and enzymes (iNOS and COX-2) were decreased significantly by PMF treatment in RAW 264.7 cells. In addition, the LPS-induced up-regulations of nuclear p65, p38, p-p38, p-ERK1/2, JNK and p-JNK proteins in RAW 264.7 cells significantly decreased by PMF, and expressions of cytosolic p65 and I kappa B were obviously up-regulated after treatment with PMF. In conclusion, we suggested that the PMF is a promising potential anti-inflammatory drug, and PMF could down-regulate expressions of pro-inflammatory cytokines and mediators by inhibiting the NF-kappa B/MAPK pathways. (C) 2015 Published by Elsevier B.V.
机译：这项研究旨在调查从年轻芦荟中分离出的芦荟苷F（PMF）的抗炎作用，并探讨可能的药理机制。通过使用角叉菜胶诱导的大鼠爪水肿试验，二甲基苯诱导的耳部水肿试验，乙酸诱导的血管通透性和棉球肉芽肿试验来评估PMF的抗炎作用。此外，通过ELISA确定促炎性细胞因子（TNF-α，IL-6和IL-1β）的释放。为了探索潜在的机制，通过定量实时PCR和蛋白质印迹测定来确定iNOS和COX-2的表达。另外，通过蛋白质印迹法测定核p65，胞质p65，IκB，p38，p-p38，p-ERK1 / 2，ERK1 / 2，JNK和p-JNK的表达。我们的结果表明，口服PMF不仅可以显着减轻大鼠的爪足水肿和小鼠的耳水肿，还可以降低小鼠的血管通透性和大鼠肉芽肿的重量。在体外，通过PMF处理在RAW 264.7细胞中，LPS诱导的促炎性细胞因子（TNF-α，IL-6，IL-1β）和酶（iNOS和COX-2）的释放显着降低。此外，PMF显着降低了LPS诱导RAW 264.7细胞中核p65，p38，p-p38，p-ERK1 / 2，JNK和p-JNK蛋白的上调，并且胞浆p65和IκB的表达PMF治疗后明显上调。总之，我们认为PMF是一种有前途的潜在抗炎药，并且PMF可以通过抑制NF-κB/ MAPK通路来下调促炎性细胞因子和介体的表达。 （C）2015由Elsevier B.V.发布

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