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首页> 外文期刊>International immunopharmacology >Guggulsterone attenuates cerulein-induced acute pancreatitis via inhibition of ERK and JNK activation
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Guggulsterone attenuates cerulein-induced acute pancreatitis via inhibition of ERK and JNK activation

机译：Guggulsterone通过抑制ERK和JNK激活来减轻青霉素诱导的急性胰腺炎

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Guggulsterone (GS), a plant steroid and a compound found at high levels in Commiphora myrrha, exhibits anti-inflammatory, anti-cancer, and cholesterol-lowering effects. However, the potential of GS to ameliorate acute pancreatitis (AP) is unknown. The aim of this study was to evaluate the effects of GS on cerulein-induced AP. AP was induced by intraperitoneally injecting supramaximal concentrations of the stable cholecystoldnin analog cerulein (50 mu g/kg) hourly for 6 h. In the GS-treated group, GS was administered intraperitoneally (10, 25, or 50 mg/kg) 1 h before the first cerulein injection. Mice were sacrificed 6 h after the final cerulein injection. Blood samples were collected to measure serum lipase levels and evaluate cytokine production. The pancreas and lung were rapidly removed for morphologic and histological examinations, flow cytometry analysis, myeloperoxidase (MPO) assay, and real-time reverse transcription-polymerase chain reaction analysis. Pretreatment with GS attenuated cerulein-induced histological damage, reduced pancreas weight/body weight ratio, decreased serum lipase levels, inhibited infiltrations of macrophages and neutrophils, and suppressed cytokine production. Additionally, GS treatment suppressed the activation of extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK) in the pancreas in cerulein-induced pancreatitis. In conclusion, our results suggest that GS attenuates AP via deactivation of ERK and JNK. (c) 2015 Elsevier B.V. All rights reserved.
机译：Guggulsterone（GS）是一种植物类固醇，是一种在Commiphora没药中含量很高的化合物，具有抗炎，抗癌和降低胆固醇的作用。但是，GS改善急性胰腺炎（AP）的潜力尚不清楚。这项研究的目的是评估GS对青霉素诱导的AP的影响。每小时腹膜内注射超最大浓度的稳定的胆囊收缩素类似物cerulein（50μg / kg），持续6 h诱导AP。在GS治疗组中，在首次注射铜蓝蛋白前1小时腹膜内给予GS（10、25或50 mg / kg）。最后一次注射青霉素后6小时，处死小鼠。收集血液样品以测量血清脂肪酶水平并评估细胞因子的产生。快速取出胰腺和肺，以进行形态学和组织学检查，流式细胞术分析，髓过氧化物酶（MPO）分析以及实时逆转录聚合酶链反应分析。 GS预处理可减轻cerulein诱导的组织学损伤，降低胰腺重量/体重比，降低血清脂肪酶水平，抑制巨噬细胞和中性粒细胞的浸润，并抑制细胞因子的产生。此外，GS治疗抑制了由小脑素诱导的胰腺炎胰腺中细胞外信号调节蛋白激酶（ERK）和c-Jun N端激酶（JNK）的活化。总之，我们的结果表明，GS通过激活ERK和JNK来减弱AP。 （c）2015 Elsevier B.V.保留所有权利。

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