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首页> 外文期刊>International immunopharmacology >Aberrant expression of RUNX3 in patients with immune thrombocytopenia
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Aberrant expression of RUNX3 in patients with immune thrombocytopenia

机译：免疫性血小板减少症患者RUNX3的异常表达

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摘要

Immune thrombocytopenia (ITP) is an autoimmune disease, characterized by dysregulation of cellular immunity. Previous studies demonstrated that immune imbalance between Th1 and Th2 was associated with the pathogenesis of ITP. Runt-related transcription factor 3 (RUNX3) is a member of the runt domain-containing family of transcription factors and plays an important role in the regulation of T cell differentiation into Th1 cells. Whether RUNX3 was involved in the pathogenesis of ITP remains unclear. In this study, 47 active ITP patients, 18 ITP with remission and 26 age and gender matched healthy control were included. Peripheral blood mononuclear cells (PBMCs) were isolated from ITP and control for isolation of RNA and plasma which were used to measure mRNA level of RUNX3 and T-box transcription factor (T-bet) by quantitative real-time PCR and interferon gamma (IFN-gamma) plasma level by ELISA. Meanwhile, protein was also extracted from PBMCs for Western blot analysis of RUNX3 expression. Our results showed a significantly higher expression of RUNX3, T-bet and plasma level of IFN-gamma in active ITP patients compared to control. No differences were observed between ITP with remission and control. Furthermore, a positive correlation of RUNX3 with T-bet was found in active ITP patients. In conclusion, aberrant expression of RUNX3 was associated with the pathogenesis of HP and therapeutically targeting it might be a novel approach in ITP treatment. (C) 2015 Elsevier B.V. All rights reserved.
机译：免疫性血小板减少症（ITP）是一种自身免疫性疾病，其特征在于细胞免疫功能异常。先前的研究表明Th1和Th2之间的免疫失衡与ITP的发病机制有关。矮子相关转录因子3（RUNX3）是含矮子域的转录因子家族的成员，在调节T细胞分化为Th1细胞方面起着重要作用。 RUNX3是否参与ITP的发病机制仍不清楚。在这项研究中，纳入了47位活跃的ITP患者，18位ITP缓解患者以及26位年龄和性别匹配的健康对照者。从ITP分离外周血单个核细胞（PBMC），并对照RNA和血浆，通过定量实时PCR和干扰素γ（IFN）测量RUNX3和T-box转录因子（T-bet）的mRNA水平。 -γ）血浆水平通过ELISA。同时，还从PBMC中提取蛋白质以用于RUNX3表达的蛋白质印迹分析。我们的结果显示，与对照组相比，活跃的ITP患者的RUNX3，T-bet和IFN-γ血浆水平明显更高。在缓解和控制的ITP之间未观察到差异。此外，在活跃的ITP患者中发现RUNX3与T-bet呈正相关。总之，RUNX3的异常表达与HP的发病机制有关，并且靶向治疗它可能是ITP治疗中的一种新方法。 （C）2015 Elsevier B.V.保留所有权利。

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