A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

Voets G.M.; Fluit A.C.; Scharringa J.; Cohen Stuart J.; Leverstein-Van Hall M.A.

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A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

机译：一组多重PCR，用于基因型检测广谱β-内酰胺酶，碳青霉烯酶，质粒介导的AmpCβ-内酰胺酶和OXAβ-内酰胺酶

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Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.

机译：在世界范围内，由于β-内酰胺酶引起的革兰氏阴性微生物对第三代头孢菌素和碳青霉烯类的耐药性是一个日益严重的问题。尽管CTX-M，TEM和SHV广谱β-内酰胺酶（ESBLs）的分布最广泛，但最近也出现了其他β-内酰胺酶家族，例如质粒介导的AmpCβ-内酰胺酶和碳青霉烯酶。在这里，我们描述了一套新的多重聚合酶链反应（PCR），其中一种扩增协议能够检测25种流行的β-内酰胺酶家族，包括ESBLs，碳青霉烯酶，质粒介导的AmpCβ-内酰胺酶和OXAβ-内酰胺酶。

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来源
《International journal of antimicrobial agents》 |2011年第4期|共4页
作者
Voets G.M.; Fluit A.C.; Scharringa J.; Cohen Stuart J.; Leverstein-Van Hall M.A.;
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正文语种 eng
中图分类抗生素;
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Carbapenemases; Extended-spectrum β-lactamases; Multiplex PCR; OXA β-lactamases; Plasmid-mediated AmpC β-lactamases;

机译：碳青霉烯酶;广谱β-内酰胺酶;多重PCR;OXAβ-内酰胺酶;质粒介导的AmpCβ-内酰胺酶;

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1. A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases [J] . Voets G.M., Fluit A.C., Scharringa J., International journal of antimicrobial agents . 2011,第4期

机译：一组多重PCR，用于基因型检测广谱β-内酰胺酶，碳青霉烯酶，质粒介导的AmpCβ-内酰胺酶和OXAβ-内酰胺酶
2. Cefotaxime for the detection of extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase and clinical characteristics of cefotaxime-non-susceptible Escherichia coli and Klebsiella pneumoniae bacteraemia [J] . MatsumuraY., YamamotoM., MatsushimaA., European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology . 2012,第8期

机译：头孢噻肟用于检测超广谱β-内酰胺酶或质粒介导的AmpCβ-内酰胺酶及头孢噻肟非敏感性大肠杆菌和肺炎克雷伯菌菌血症的临床特征
3. Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM) [J] . Gaelle Cuzon1 Thierry Naas1* Pierre Bogaerts2 Youri Glupczynski2 and Patrice Nordmann1 Journal of Antimicrobial Chemotherapy . 2012,第8期

机译：评估用于快速检测超广谱β-内酰胺酶（TEM，SHV和CTX-M），质粒介导的头孢菌素酶（CMY-2-like，DHA，FOX，ACC-1，ACT / MIR和CMY）的DNA芯片的评估-1-样/ MOX）和碳青霉烯酶（KPC，OXA-48，VIM，IMP和NDM）
4. Detection and Discrimination of Extended-Spectrum beta-lactamase (ESBL) Producing Bacteria by MALDI-TOF-MS [C] . Ian Edwards, Edina Chiriseri, Marilena Ioannou, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：MALDI-TOF-MS的扩展β-内酰胺酶（ESBL）产生细菌的检测和辨别
5. Prevalence of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae with Focus on the Molecular Characterization of ESBL- and AmpC β-Lactamase-Producing Escherichia coli Isolated in Canadian Hospitals from 2005-2009 [D] . Simner, Patricia Jeanne 2011

机译：2005-2009年在加拿大医院分离的产广谱β-内酰胺酶肠杆菌的流行情况，侧重于生产ESBL和AmpCβ-内酰胺酶的大肠杆菌的分子表征
6. Occurrence of Extended-Spectrum and AmpC Beta-Lactamases in Bloodstream Isolates of Klebsiella pneumoniae: Isolates Harbor Plasmid-Mediated FOX-5 and ACT-1 AmpC Beta-Lactamases [O] . Philip E. Coudron, Nancy D. Hanson, Michael W. Climo 2003

机译：肺炎克雷伯菌的血流分离物中出现广谱和AmpCβ-内酰胺酶：分离港口质粒介导的FOX-5和ACT-1 AmpCβ-内酰胺酶
7. A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases [O] . Voets, Guido M., Fluit, A.C., Scharringa, Jelle, 2011

机译：一组多重PCR，用于对广谱β-内酰胺酶，碳青霉烯酶，质粒介导的AmpCβ-内酰胺酶和OXAβ-内酰胺酶进行基因型检测

A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

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