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首页> 外文期刊>International journal of antimicrobial agents >Chlorhexidine-impregnated PEM/THFM polymer exhibits superior activity to fluconazole-impregnated polymer against Candida albicans biofilm formation
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Chlorhexidine-impregnated PEM/THFM polymer exhibits superior activity to fluconazole-impregnated polymer against Candida albicans biofilm formation

机译：氯己定浸渍的PEM / THFM聚合物对氟康唑浸渍的聚合物具有更好的抗白色念珠菌生物膜形成的活性

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Biofilm-associated infections represent a major challenge for biomaterials. Methods to alter the chemical characteristics of biomaterials offer an attractive solution for enhanced microbial control. The aim of this study was to investigate the efficacy of a poly(ethyl methacrylate)/tetrahydrofurfuryl methacrylate (PEM/THFM) acrylic model impregnated with fluconazole (FLU) or chlorhexidine (CHX) in preventing Candida biofilm formation in vitro. PEM/THFM disks impregnated with CHX (n = 50) or FLU (n = 50) and drug-free control disks (n = 50) were infected with Candida albicans ATCC 90028. Disks were incubated for 2, 7, 14, 21 or 28 days at 37 °C and the biofilm biomass and metabolic activity was quantified at each time point using crystal violet staining and XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] reduction assay. FLU disks exhibited poor overall biofilm inhibitory characteristics, with mean metabolic and biomass inhibition of 12.6% and 8.8%, respectively. Conversely, CHX disks were highly effective, significantly inhibiting biofilm development by 75% (P ≤ 0.001) and its metabolism by 84% (P ≤ 0.001) for all time points tested. The notable efficacy of CHX against C. albicans biofilms is a promising outcome to overcome the side effects and poor relative activity of conventional antifungal agents against Candida biofilms. These findings indicate that impregnation of PEM/THFM with antimicrobials has potential as a treatment modality for denture stomatitis.
机译：与生物膜相关的感染是生物材料面临的主要挑战。改变生物材料化学特性的方法为增强微生物控制提供了有吸引力的解决方案。这项研究的目的是研究浸渍有氟康唑（FLU）或洗必泰（CHX）的聚甲基丙烯酸乙酯/甲基丙烯酸四氢糠酯（PEM / THFM）丙烯酸酯模型在体外预防念珠菌生物膜形成的功效。用白色念珠菌ATCC 90028感染用CHX（n = 50）或FLU（n = 50）浸渍的PEM / THFM盘和无毒对照盘（n = 50）。将盘孵育2、7、14、21或在37°C下放置28天，并使用结晶紫染色和XTT [2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑鎓对每个时间点的生物膜生物量和代谢活性进行定量-5-甲酰苯胺]还原法。 FLU盘显示出较差的整体生物膜抑制特性，平均代谢和生物量抑制分别为12.6％和8.8％。相反，CHX盘非常有效，在所有测试时间点上，其生物膜的发育均显着抑制了75％（P≤0.001），其新陈代谢则抑制了84％（P≤0.001）。 CHX对抗白色念珠菌生物膜的显着功效是克服常规抗真菌剂针对念珠菌生物膜的副作用和较差相对活性的有希望的结果。这些发现表明，用抗菌素浸渍PEM / THFM具有作为义齿口腔炎的治疗方法的潜力。

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