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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Effect of organic solvents on the conformation and interaction of catalase and anticatalase antibodies
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Effect of organic solvents on the conformation and interaction of catalase and anticatalase antibodies

机译:有机溶剂对过氧化氢酶和抗过氧化氢酶抗体构象和相互作用的影响

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摘要

Effect of six organic solvents-methanol, ethanol, propanol, dimethyl sulphoxide (DMSO), N,N-dimethyl formamide (DMF), and glycerol on the conformation and interaction of catalase and anticatalase antibodies were studied with the aim of identifying the solvents in which antigen-antibody interactions are strong. The antigen binding activity of the antibodies in the various organic solvents increased in the following order: ethanol < methanol < no organic solvent < propanol < DMSO < DMF < glycerol. The structure of both the antibody and the antigen molecule was affected significantly in 40% concentration of the organic solvents used in this study. Catalase activity was inhibited in DMSO. However, the enzyme was activated in DMF upto about 50% of its concentration. (c) 2006 Elsevier B.V. All rights reserved.
机译:研究了六种有机溶剂-甲醇,乙醇,丙醇,二甲基亚砜(DMSO),N,N-二甲基甲酰胺(DMF)和甘油对过氧化氢酶和抗过氧化氢酶抗体的构象和相互作用的作用,目的是鉴定哪些抗原-抗体相互作用强。抗体在各种有机溶剂中的抗原结合活性按以下顺序增加:乙醇<甲醇<无有机溶剂<丙醇

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