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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >ON THE STRUCTURE OF AGGREGATED KAPPA-CARRAGEENAN HELICES - A STUDY BY CRYO-TEM, OPTICAL ROTATION AND VISCOMETRY
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ON THE STRUCTURE OF AGGREGATED KAPPA-CARRAGEENAN HELICES - A STUDY BY CRYO-TEM, OPTICAL ROTATION AND VISCOMETRY

机译:聚集的卡拉胶-角叉菜胶结构的研究-透射电镜,光学旋转和粘滞性研究

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摘要

Cryo-transmission electron microscopy (cryo-TEM), viscometry and optical rotation have been used to study the microstructure of dilute solutions of helical K-carrageenan under varied electrolyte composition (0.1 M of NaI, CsI and mixtures of the two salts). Microfibers (300-400 nm in length) were observed above a critical cesium content, while below that no microstructure was seen in the micrographs. A drastic increase in the specific viscosity and an onset of thermal hysteresis in the optical rotation above the same critical cesium content provide clear evidence of aggregate formation. This leads to the conclusion that the observed microfibers are aggregates consisting of several helices. At high proportions of cesium, further aggregation of the microfibers was observed by cryo-TEM. [References: 23]
机译:低温透射电镜(cryo-TEM),粘度测定法和旋光性已用于研究在不同电解质组成(0.1 M NaI,CsI和两种盐的混合物)下螺旋K-角叉菜胶稀溶液的微观结构。在临界铯含量以上观察到超细纤维(长度为300-400 nm),而在显微照片下未观察到超细纤维。在相同的临界铯含量以上,比粘度的急剧增加和旋光性的热滞现象的开始,提供了明显的聚集体形成证据。由此得出结论,观察到的微纤维是由几个螺旋组成的聚集体。在铯含量高的情况下,通过冷冻TEM观察到微纤维的进一步聚集。 [参考:23]

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