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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Anomalous colloidal stability of protein coated polystyrene latex beads studied by small angle light scattering
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Anomalous colloidal stability of protein coated polystyrene latex beads studied by small angle light scattering

机译：小角度光散射研究蛋白包被的聚苯乙烯乳胶珠的胶体反常稳定性

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摘要

Results of small angle laser light scattering experiments carried out on polystyrene latex beads coated with lysate of Plasmodium falciparum (Pf) antigen (Ag) and human seropositive sera (10:1 ratio) are reported for various NaCl concentrations (0-300 mM). The protein coated beads showed time-dependent coagulation. The normalised intensity of scattered light I-s(t)I-o showed I-s(t)/I-o =1+(Gamma t)(delta) behaviour with the coagulation rate, Gamma and exponent, delta showing anomalous dependence on NaCl concentration. The coagulation rate exhibited strong increase up to NaCl concentration of 50 mM, above this and up to 300 mM the coagulation rate was found to remain independent of NaCl concentration yielding non-DLVO behaviour. The same was true for delta which increased from 1.04 +/- 0.06 to 6.94 +/- 0.07 as NaCl concentration was raised from 0 to 50 mM. Above 50 mM it remained constant with delta = 6.94 +/- 0.07. Results are discussed through Smoluchowski aggregation kinetics and theoretical construction of interparticle interaction potentials relevant to our problem. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 32]
机译：对于各种浓度的NaCl（0-300 mM），据报道对涂有恶性疟原虫（Pf）抗原（Ag）和人血清阳性血清（10：1比例）的聚苯乙烯乳胶珠进行的小角度激光散射实验的结果。蛋白质包被的珠子显示出时间依赖性的凝结。散射光I-s（t）I-o的归一化强度显示I-s（t）/ I-o = 1 +（Gamma t）δ行为，其凝固速率，Gamma和指数，δ显示出对NaCl浓度的反常依赖。凝结速率在直至50 mM的NaCl浓度下均显示出强劲的增加，在此之上和直至300 mM，发现凝结速率与NaCl浓度无关，从而产生非DLVO行为。当NaCl浓度从0mM增加到50mM时，δ也从1.04 +/- 0.06增加到6.94 +/- 0.07，同样如此。高于50 mM时，它保持恒定，Δ= 6.94 +/- 0.07。通过Smoluchowski聚集动力学和与我们问题相关的粒子间相互作用势的理论构造来讨论结果。 （C）2000 Elsevier Science B.V.保留所有权利。 [参考：32]

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