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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Chitosan-Si02-multiwall carbon nanotubes nanocomposite: A novel matrix for the immobilization of creatine amidinohydrolase
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Chitosan-Si02-multiwall carbon nanotubes nanocomposite: A novel matrix for the immobilization of creatine amidinohydrolase

机译：壳聚糖-SiO 2-多壁碳纳米管纳米复合材料：固定化肌酸a水解酶的新型基质

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摘要

A matrix made up of chitosan-Si0_2-multiwall carbon nanotubes (CHIT-Si0_2-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-Si0_2-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-Si0_2-MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT-Si0_2-MWNT5 as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.58 mM and 83.16 mg/cm~2, respectively; indicating CHIT-Si0_2-MWCNTs nanocomposite matrix has a high affinity to immobilize CAH enzyme.
机译：制备了由壳聚糖-SiO 2-多壁碳纳米管（CHIT-SiO 2 -MWCNTs）纳米复合材料组成的基质，以研究肌酸a水解酶（CAH）的固定化。使用戊二醛作为接头，将CAH酶与CHIT-SiO_2-MWCNTs基质共价固定。以傅立叶变换红外光谱（FTIR），扫描电子显微镜（SEM）和循环伏安法（CV）为特征，以CHIT-SiO 2 -MWNT5为参考，对所得的CAH / CHIT-SiO 2 -MWCNTs生物基质进行了表征。研究了各种参数对基质内CAH酶活性的影响，包括pH，温度和时间。计算得出CAH酶的Michaelis-Menten常数和表观活性分别为0.58 mM和83.16 mg / cm〜2。说明CHIT-SiO_2-MWCNTs纳米复合材料基质对CAH酶具有较高的亲和力。

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