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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Physicochemical and biological activity study of genipin-crosslinked chitosan scaffolds prepared by using supercritical carbon dioxide for tissue engineering applications
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Physicochemical and biological activity study of genipin-crosslinked chitosan scaffolds prepared by using supercritical carbon dioxide for tissue engineering applications

机译：超临界二氧化碳制备的京尼平交联壳聚糖支架的理化和生物活性研究在组织工程中的应用

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摘要

The present study deals with the preparation of porous chitosan scaffolds by using a greener technique i.e., supercritical carbon dioxide (sc.CO2). 0.2Mchitosan (CS) solution in aqueous acetic acid was treated with 4% (w/v) genipin solution; the resulting hydrogels were subjected to solvent exchange prior to the final treatment procedures. Their morphology, pore structure, and physical properties were characterized by thermal analysis, X-ray diffractogram (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and the specific surface areas and porosimetry of scaffolds were determined by using N2 adsorption. The biological activity of scaffolds was investigated by immersing them into 1.5× simulated body fluid (SBF) and cellular attachment study was assessed using Alamar Blue assay by seeding MG63 osteoblastic cells onto the scaffolds. The sc.CO2 assisted chitosan scaffold prepared by using green chemistry approach is highly pure and bioactive and can be served as a potential material for tissue engineering applications.
机译：本研究涉及通过使用绿色技术即超临界二氧化碳（sc.CO2）制备多孔壳聚糖支架。用4％（w / v）的Genipin溶液处理在乙酸水溶液中的0.2Mchitosan（CS）溶液;在最终处理程序之前，将得到的水凝胶进行溶剂交换。通过热分析，X射线衍射图谱（XRD），扫描电子显微镜（SEM），透射电子显微镜（TEM）表征了它们的形态，孔结构和物理性质，并使用N2测定了支架的比表面积和孔隙率吸附。通过将支架浸入1.5x模拟体液（SBF）中来研究支架的生物活性，并通过将MG63成骨细胞接种到支架上，使用Alamar Blue分析评估细胞附着研究。使用绿色化学方法制备的sc.CO2辅助壳聚糖支架具有很高的纯度和生物活性，可作为组织工程应用的潜在材料。

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