...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >An evidence for the equilibrium unfolding intermediates of ribonuclease A by tritium labeling method
【24h】

An evidence for the equilibrium unfolding intermediates of ribonuclease A by tritium labeling method

机译：tri标记法证明核糖核酸酶A平衡展开中间体的证据

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A formation of a molten globule in the unfolding of ribonuclease A could be considered as an evidence supporting a hypothesis on the existence of such intermediates on the pathway of a protein folding. Using a novel technique (tritium labeling method) we have showed that the ribonuclease A equilibrium unfolding in urea and guanidinium chloride (GuCl) solutions proceeds through a formation of intermediates whose properties (compactness, retention of the larger pan hydrophobic core, secondary structure, and native-like folding pattern) correspond to the fundamental characteristics of the molten globule state. The both intermediates are the "wet" molten globules (the globule interior contains the water molecules). The results reveal the noticeable distinctions in intermediates structure, first of all, in the extent of their compactness. The urea intermediate is less compact than that in GuCl. It is shown that the refolding of the protein denatured by GuCl results in the formation of the intermediate which enzyme activity is virtually the same as the activity of the native protein. (c) 2006 Elsevier B.V. All rights reserved.
机译：核糖核酸酶A展开时形成的熔融小球可被视为支持有关蛋白质折叠路径上此类中间体存在的假设的证据。使用一种新技术（tri标记法），我们已经表明，核糖核酸酶A在尿素和氯化胍（GuCl）溶液中的平衡解构通过中间体的形成而进行，这些中间体的性质（紧凑性，较大的泛疏水核的保留，二级结构和天然的折叠模式）对应于熔融小球状态的基本特征。两种中间体都是“湿”熔融小球（小球内部含有水分子）。结果表明，中间体结构首先在紧凑性方面有明显的区别。尿素中间体的致密性不如GuCl。已经表明，由GuC1变性的蛋白质的重折叠导致形成中间体，该中间体的酶活性实际上与天然蛋白质的活性相同。 （c）2006 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号