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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >EXAMINATION OF POLYPEPTIDE BETA-SHEET STRUCTURE IN SOLUTIONS AND THIN LAYERS - DETERMINATION OF THE CONCENTRATION AND THE CRITICAL AGGREGATION CONCENTRATION USING A CYANINE DYE AS SENSOR
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EXAMINATION OF POLYPEPTIDE BETA-SHEET STRUCTURE IN SOLUTIONS AND THIN LAYERS - DETERMINATION OF THE CONCENTRATION AND THE CRITICAL AGGREGATION CONCENTRATION USING A CYANINE DYE AS SENSOR

机译:在溶液和薄层中检测多肽β-片状结构-以花青染料为传感器测定浓度和临界聚集浓度

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摘要

In the visible spectra of some cyanine dyes a bathochromic shift of the dye monomer band was observed on the preconditions that: (1) beta-sheet containing polypeptides (denotes also proteins) were presented; and (2) these polypeptides were embedded in a layer or aggregated in solution. The band with the polypeptides which contained only the alpha-helix did not shift. In several cases the absorbance lifetime of the shifted band was limited. This was caused by dye self-association at the polypeptide surface, but there were enough quantities for this lifetime to obtain exact analytical measurements. These were executed quantitatively (100-20% beta-sheet), qualitatively (to about 10% beta-sheet) and moreover for the determination of the 'critical aggregation concentration' (cac). The applications of the dye sensor in biophysics, medicine and pharmacy were discussed. (C) 1997 Elsevier Science B.V. [References: 19]
机译:在某些花菁染料的可见光谱中,在以下条件下观察到染料单体带的红移:(1)出现了含有多肽的β-折叠(也表示蛋白质); (2)将这些多肽包埋在一层中或在溶液中聚集。具有仅包含α-螺旋的多肽的条带不移动。在某些情况下,移位谱带的吸收寿命受到限制。这是由于多肽表面的染料自缔合引起的,但是在此寿命中有足够的量以获得准确的分析测量结果。定量(100-20%beta-sheet),定性(至约10%beta-sheet)执行这些操作,此外还用于确定“关键聚集浓度”(cac)。讨论了染料传感器在生物物理,医学和药学中的应用。 (C)1997 Elsevier Science B.V. [参考:19]

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