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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >The fine structure of cassava starch amylopectin. Part 2: Building block structure of clusters
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The fine structure of cassava starch amylopectin. Part 2: Building block structure of clusters

机译：木薯淀粉支链淀粉的精细结构。第2部分：集群的构建块结构

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The aim of this work was to analyse the organization of unit chains inside clusters of cassava amylopectin β-Limit dextrins of the clusters and partly fragmented clusters (sub-clusters) were isolated previously [Laohaphatanaleart et al., Int. J. Biol. Macromol. (2010) doi:10.1016/j.ijbiomac.2010.01.0049] and were now hydrolysed extensively with the α-amylase (liquefying type) of Bacillus subtilis into small, branched building blocks. The blocks were size-fractionated and characterized structurally. The smallest blocks predominated in the clusters. They were single branched and possessed a degree of polymerization (DP) of 5-9. Blocks with DP 10-15 were double branched and constituted the second largest group. The clusters of cassava amylopectin, which were of rather uniform size, possessed typically 7-9 building blocks, and all clusters contained similar size-distributions of the blocks. The inter-block chain length was 7-8 residues. The possible mode of attack by the enzyme between the building blocks is discussed. A model of the building block organization in the clusters is presented, in which the structural roles of different sub-groups of clustered chains are suggested. A three-dimensional model suggests a possible organization of the building blocks inside the amorphous lamellae in the granular starch.
机译：这项工作的目的是分析木薯支链淀粉的β-极限糊精簇内的单元链的组织，并且先前分离出部分片段化的簇（亚簇）[Laohaphatanaleart等，国际。 J.Biol。大分子（2010）doi：10.1016 / j.ijbiomac.2010.01.0049]，现在已被枯草芽孢杆菌的α-淀粉酶（液化型）广泛水解成小的分支结构块。将该块按大小分级并在结构上进行表征。簇中最小的块占主导。它们是单支链的并且具有5-9的聚合度（DP）。 DP 10-15的嵌段是双分支的，构成第二大类。木薯支链淀粉的大小相当均匀，通常具有7-9个构建基块，并且所有聚类均包含相似的块大小分布。嵌段间链长为7-8个残基。讨论了酶在结构单元之间可能的进攻方式。提出了集群中构建块组织的模型，其中提出了集群链不同子组的结构角色。三维模型表明，颗粒状淀粉中无定形薄片内部的结构单元可能会组织起来。

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