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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Synthesis and characterization of hemoglobin conjugates with antioxidant enzymes via poly(ethylene glycol) cross-linker (Hb-SOD-CAT) for protection from free radical stress
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Synthesis and characterization of hemoglobin conjugates with antioxidant enzymes via poly(ethylene glycol) cross-linker (Hb-SOD-CAT) for protection from free radical stress

机译：通过聚乙二醇交联剂（Hb-SOD-CAT）与抗氧化酶的血红蛋白结合物的合成和表征，以防止自由基应激

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Hemoglobin (Hb) conjugated with the antioxidant enzymes (SOD and CAT), by employing dicarboxymethylated poly(ethylene glycol), was designed for protection of hemoglobin against free radicals. In this study, the conjugation process was confirmed by employing SDS-PAGE and SEC techniques. The average molecular weight of the conjugates was estimated to be around 1000. kDa. The enzymatic activities of the SOD and CAT in the conjugates (Hb-SOD-CAT) after conjugation were found to retain greater than 70% and 90% of the original bioactivity. Results show that antioxidant enzymes helped minimize methemoglobin (non-carrier of oxygen) formation during the conjugation process and also during storage at 4 °C over a period of 1 month. In summary, the optimized (1:10 Hb/PEG) crosslinked conjugates with antioxidant enzymes showed protective properties from severe free radical stresses when incubated with hydrogen peroxide (0.1 and 1. mM) and xanthine (1. mM)/xanthine oxidase (10 and 20. mU/ml) system
机译：通过使用二羧甲基化聚乙二醇，与抗氧化酶（SOD和CAT）结合的血红蛋白（Hb）被设计用于保护血红蛋白免受自由基的侵害。在这项研究中，通过使用SDS-PAGE和SEC技术证实了偶联过程。缀合物的平均分子量估计为约1000kDa。发现结合后SOD和CAT在结合物中的酶促活性（Hb-SOD-CAT）保留了原始生物活性的70％和90％以上。结果表明，抗氧化酶有助于在缀合过程中以及在4°C下储存1个月的过程中将高铁血红蛋白（非氧气的载体）的形成降至最低。总而言之，与过氧化氢（0.1和1. mM）和黄嘌呤（1. mM）/黄嘌呤氧化酶（10）孵育后，具有抗氧化酶的优化（1:10 Hb / PEG）交联缀合物具有免受严重自由基胁迫的保护性能。和20. mU / ml）系统

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