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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Protective effects of sulfated chitooligosaccharides against hydrogen peroxide-induced damage in MIN6 cells
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Protective effects of sulfated chitooligosaccharides against hydrogen peroxide-induced damage in MIN6 cells

机译：硫酸壳寡糖对过氧化氢诱导的MIN6细胞损伤的保护作用

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摘要

Sulfated chitooligosaccharides (COS-S) with different degrees of substitution (DS) were obtained by the chlorosulfuric acid/pyridine method. Protective effects of COS-S against hydrogen peroxide (H _2O _2)-induced damage were investigated in pancreatic β-cells MIN6 cell line. The cell viability, morphology, insulin contents, malondialdehyde (MDA) inhibition, lactate dehydrogenase (LDH) release and the levels of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidise (GPx) were evaluated under oxidative damage by 150μM H _2O _2 for 6h. COS-S did not show any harmful or inhibitory effect on cell growth at concentrations ranging from 0.1 to 0.5mg/ml. While COS-S could enhance the cell viability, decrease the production of ROS, and reduce the MDA level as well as LDH level in oxidative damaged β-cells by being an antioxidant. The underlining mechanisms of protective effects of COS-S are partly due to the enhancement of antioxidant enzyme activity and inhibition of intracellular ROS production, along with suppressing MIN6 cell apoptosis subsequent to the amelioration of ROS. Moreover, increased DS might contribute to the defense mechanisms against H _2O _2-induced oxidative damage in MIN6 cells. These results indicated that the antioxidant properties of COS-S hold great potential for the oxidative diseases treatment, and the sulfate content of polysaccharides made great role in regulating antioxidant activities.
机译：通过氯硫酸/吡啶法获得了不同取代度的硫酸化壳寡糖（COS-S）。在胰腺β细胞MIN6细胞系中研究了COS-S对过氧化氢（H _2O _2）诱导的损伤的保护作用。通过氧化损伤评估了细胞活力，形态，胰岛素含量，丙二醛（MDA）抑制，乳酸脱氢酶（LDH）释放以及包括过氧化氢酶（CAT），超氧化物歧化酶（SOD）和谷胱甘肽过氧化酶（GPx）等抗氧化酶的水平150μMH _2O _2持续6h。 COS-S在0.1至0.5mg / ml的浓度范围内对细胞生长没有显示出任何有害或抑制作用。 COS-S是抗氧化剂，可以增强氧化损伤的β细胞的细胞活力，减少ROS的产生，并降低MDA含量和LDH含量。 COS-S保护作用的强调机制部分是由于抗氧化剂酶活性的增强和细胞内ROS的抑制，以及抑制ROS改善后的MIN6细胞凋亡。此外，增加的DS可能有助于抵御MIN6细胞中H _2O _2诱导的氧化损伤的防御机制。这些结果表明，COS-S的抗氧化性能在治疗氧化性疾病方面具有巨大潜力，多糖中硫酸盐的含量对调节抗氧化活性具有重要作用。

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