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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Bio-waste derived dialdehyde cellulose ethers as supports for alpha-chymotrypsin immobilization
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Bio-waste derived dialdehyde cellulose ethers as supports for alpha-chymotrypsin immobilization

机译：生物废物衍生的二醛纤维素醚作为α-胰凝乳蛋白酶固定化的载体

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摘要

Enzyme immobilization is an important technique to enhance stability, storability and reusability of enzymes. In the present work, pine needles, a forest bio-waste, were used as a feedstock of cellulose to synthesize new materials as supports for immobilization of alpha-chymotrypsin (CT) enzyme. The extracted cellulose from pine needles was etherified with different alkyl bromides (RBr) and etherified products were further modified to dialdehyde via oxidation with NaIO4 to get the desired products, dialdehyde cellulose ethers (R-O-cell-CH=O). CT was then covalently immobilized onto as-synthesized dialdehyde cellulose ethers via Schiff-base formation, i.e., imine linkage. The synthesized products and enzyme immobilization were confirmed by different characterization techniques and the activity assay of the free and the immobilized CT was carried out using standard protocol with variation of different parameters such as temperature, pH and substrate concentration. The storage stability and reusability of the immobilized CT were also investigated. CT activity was also studied in simulated physiological conditions in the artificial gastric fluid and artificial intestinal fluid. Artificial neural network (ANN) model was employed to correlate the relationship with% relative activity and time, temperature and pH affecting enzyme activity. A good correlation of experimental data was predicted by ANN model. (C) 2015 Elsevier B.V. All rights reserved.
机译：固定酶是增强酶的稳定性，可存储性和可重复使用性的重要技术。在目前的工作中，松针（一种森林生物废物）被用作纤维素的原料，以合成新材料作为固定化α-胰凝乳蛋白酶（CT）酶的载体。从松针中提取的纤维素用不同的烷基溴（RBr）醚化，醚化的产物经NaIO4氧化进一步改性为二醛，得到所需的产物二醛纤维素醚（R-O-cell-CH = O）。然后，通过席夫碱形成即亚胺键合将CT共价固定在合成的二醛纤维素醚上。通过不同的表征技术确认了合成产物和酶的固定化，并使用标准规程在温度，pH和底物浓度等不同参数的变化下进行游离和固定化CT的活性测定。还研究了固定式CT的存储稳定性和可重复使用性。在模拟的生理条件下，在人工胃液和人工肠液中还研究了CT活性。人工神经网络（ANN）模型被用来关联与相对活性百分比和时间，温度和pH值影响酶活性的关系。 ANN模型预测了实验数据的良好相关性。 （C）2015 Elsevier B.V.保留所有权利。

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