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首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Generation of nanoparticles from technical powder of the insecticidal bacterium Bacillus thuringiensis var. kurstaki for improving efficacy
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Generation of nanoparticles from technical powder of the insecticidal bacterium Bacillus thuringiensis var. kurstaki for improving efficacy

机译：从杀虫苏云金芽孢杆菌变种的工业粉末中生成纳米颗粒。库尔斯塔基提高疗效

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摘要

The technical powder of a local isolate of Bacillus thuringiensis (Bt) var. kurstaki namely DOR Bt-1 was subjected to high pressure homogenisation (HPH) to obtain nanoparticles (NPs) ranging 32-1,106 nm as determined through dynamic light scattering. NPs of Bt were also characterised for shape and size through scanning electron microscopy, field emission scanning electron microscopy and transmission electron microscopy that helped in visualising the smallest nanoparticles, i.e., less than even 50 nm. The larger particles comprised mainly Bt spores with a length and width of 1,139 and 813 nm respectively. HPH showed little effect on the spore viability. NPs had a zeta potential above +30 and were stable, resulted in higher mortality of Helicoverpa armigera larvae at a lower dose. Alkali solubilisation of the NPs resulted in higher values of protein and endotoxin quantified through Lowry's method and ELISA respectively. This is indicative of an increased solubility of the toxin in the midgut alkaline fluid of the insect leading to higher mortality coupled with quicker kill. Thus Bt as NPs has brighter prospects for insect pest management with increased speed of kill at lower doses.
机译：苏云金芽孢杆菌（Bt）var本地分离株的技术粉末。通过动态光散射测定，对kurstaki（即DOR Bt-1）进行高压均质化（HPH），以获得32-1,106 nm的纳米粒子（NPs）。还通过扫描电子显微镜，场发射扫描电子显微镜和透射电子显微镜表征了Bt的NP的形状和大小，这有助于可视化最小的纳米颗粒，即小于50nm。较大的颗粒主要包括长度和宽度分别为1,139和813 nm的Bt孢子。 HPH对孢子活力几乎没有影响。 NPs的Zeta电位高于+30，并且稳定，因此在较低剂量下导致棉铃虫幼虫的死亡率较高。 NPs的碱溶解导致分别通过Lowry方法和ELISA定量的蛋白质和内毒素值更高。这表明毒素在昆虫的中肠碱性液中的溶解度增加，导致更高的死亡率和更快的杀灭力。因此，作为NP的Bt具有较低的杀灭速度，具有更高的杀虫速度，可用于虫害防治。

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